三七皂苷清除活性氧自由基及对DNA氧化损伤保护作用研究  

Effects of Panax notoginseng Saponins on Antioxidation and Preventing DNA Damage Caused by Hydroxyl Radical

作　　者：高云涛[1] 张文斌 杨利荣[1] 王雪梅[1] 杨益林[1] 

机构地区：[1]云南民族大学化学与生物技术学院,云南昆明650031 [2]云南省文山州三七科学技术研究所,云南文山663000

出　　处：《中药材》2008年第9期1399-1402,共4页Journal of Chinese Medicinal Materials

摘　　要：利用Fenton反应、邻苯三酚自氧化反应及光照核黄素产生活性氧自由基,紫外-可见分光光度法研究了三七总皂苷在体外对活性氧自由基的清除作用。通过硫代巴比妥分光光度法研究三七总皂苷对.OH引起的DNA氧化损伤的保护作用。实验结果表明三七总皂苷对.OH、O2.-自由基有明显的清除作用,并能显著抑制.OH对DNA氧化损伤。Scavening effects of panax notoginseng saponins on active oxygen species in the systems of Fenton reaction, autoxidation of pyrogallol and riboflavine photosensitization were investigated by .the mean of spectrophotometry. The inhibition ofpanax notoginseg saponins on the damage of DNA chain induced by hydroxyl radical by panax notoginseng saponins was observed by the mean of gelose gel electrophoresi and thiobartital spectrophotometry. The results showed that panax notoginseng saponins could scaven · OH, O2^- efficiently and protect DNA chains from being damaged by hydroxyl radical.

关 键 词：三七总皂苷 活性氧自由基 DNA损伤 

分 类 号：R285.5[医药卫生—中药学]