大孔吸附树脂分离纯化三七花蕾总皂苷的研究  

Studies on Separation and Purification of Total Saponin from Flower buds of Panax notoginseng by Macroporous Resins

作　　者：魏莉[1] 周浩[1] 

机构地区：[1]上海中医药大学,上海201203

出　　处：《中药材》2008年第9期1418-1421,共4页Journal of Chinese Medicinal Materials

基　　金：上海市高新技术产业科技开发基金资助项目(沪创新J-58)

摘　　要：目的:研究大孔吸附树脂分离纯化三七花蕾中总皂苷的工艺条件及参数。方法:以三七花蕾总皂苷的吸附量和洗脱率为指标,筛选大孔吸附树脂,并研究所选树脂分离纯化三七花蕾总皂苷的吸附和洗脱条件。结果:AB-8树脂对三七花蕾总皂苷有较好的吸附分离性能。最佳工艺条件为:上样浓度为0.2 g生药/m l,以3BV 70%乙醇溶液洗脱,吸附及洗脱流速均为2BV/h,洗脱液蒸干,即得纯化的三七花蕾总皂苷提取物。纯化后三七花蕾总皂苷含量为87.60%。结论:AB-8大孔吸附树脂分离纯化三七花蕾中总皂苷是可行的。Objective ： To optimize the technical parameters of separation and purification of total saponin from flower buds of Panax notoginseng by macroporous resins. Methods ： Based on absorptive capacity and elution efficiency of total saponin from flower buds of Panax notoginseng, the best type of resin and the conditions to separate and purify total saponin by macroporous resins were optimized. Results： The AB-8 resin was chosen, washed with 3 BV of 70% ethanol, and with the absorption and elution velocity of flow 2BV/h. The optimal concentration of the raw material was 0. 2 g/ml. The purity of total saponin obtained was 87.60%. Conclusion： Using AB-8 resin to separate and purify total saponin from flowers of Panax notoginseng is feasible.

关 键 词：三七花蕾 总皂苷 大孔吸附树脂 分离纯化 

分 类 号：R284.2[医药卫生—中药学]