三种植物次生物质对B型烟粉虱羧酸酯酶活性及其对药剂敏感度的影响  

Effects of Three Allelochemcals on the Carboxylesterase Activity and its Susceptibility to Insecticides in Bemisia tabaci Biotype B

作　　者：张爱萍[1] 宋敦伦[1] 史雪岩[1] 梁沛[1] 高希武[1] 

机构地区：[1]中国农业大学昆虫学系,北京100193

出　　处：《农药学学报》2008年第3期292-296,共5页Chinese Journal of Pesticide Science

基　　金：国家重点基础研究发展规划(973计划)项目(2002CB111407)

摘　　要：采用人工饲料添加法，研究了不同浓度的槲皮素、2-十三烷酮和葫芦素B3种植物次生物质对B型烟粉虱Bemisiatabaci羧酸酯酶（Care）活性的影响，同时比较研究了用3种植物次生物质处理后敌敌畏、脱叶磷、灭多威、乐果和氧乐果5种药剂对CarE的抑制中浓度（Ⅰ50）的变化。结果表明：0．01—1．0mg／mL的槲皮素对B型烟粉虱成虫CarE活性均具有明显的诱导增加作用，最高为对照的4．32倍；0．1和0．5mg／mL的2-十三炕酮处理使CarE活性比对照分别下降了22％和58％；0．75～30．0mg／L的葫芦素B对烟粉虱CarE活性均表现为抑制作用；用1．0mg／mL的槲皮素处理24h后，敌敌畏和脱叶磷对烟粉虱CarE的Ⅰ50轴值分别增加为对照的20．05和3．16倍；3．75mg／L的葫芦素B处理后，敌敌畏和灭多威对CarE的Ⅰ50值分剐增加为对照的25．94、2．45倍；1．0mg／mL的2-十三烷酮处理后敌敌畏、脱叶磷和乐果对CarE的Ⅰ50值分别降低了89％、83％和90％。The effects of quercetin, 2-tridecanone and cucurbitacin B on the specific activity of carboxylesterase （CarE） in the tobacco whitefly,Bemisia tabaci （Gennadius） type B were investigated by feeding on artificial diet. The sensitivities of CarE to dichlorvos, methomyl, dimethoate, omethoate and S, S, S-tributyl phosphorotrithioate（ DEF）, an inhibitor of CarE, were also determined. After treated with quercetin ranged from 0.01 mg/mL to 1. 0 mg/mL for 24 h, the specific activity of CarE increased obviously and the highest was 4.32-fold higher than the control. The specific activity of CarE was partially inhibited by 22% and 58%, respectively in the 0. 1 and 0. 5 mg/mL 2-tridecanone treatments. The cucurbitacin B ranged from 0.75 to 30.0 mg/L inhibited activity of CarE obviously. The I50 values of dichlorvos and DEF to the CarE from B. tabaci treated with 1.0 mg/L quercetin were respectively 20.05- and 3.16-fold higher than the control. When treated with 3.75 mg/L of cucurbitacin B for 24 h,the 150 values of dichlorvos and methomyl to the Care were 25.94 - and 2.45 - fold higher than the control,respectively. While when treated with 1. 0 mg/mL 2-tridecanone for 24 h, the I50 values of dichlorvos,DEF and dimethoate to the CarE were decreased by 89%,83% and 90%,respectively.

关 键 词：烟粉虱 槲皮素 2-十三烷酮 葫芦素B 羧酸酯酶 杀虫剂 

分 类 号：S482.39[农业科学—农药学] S481.1[农业科学—植物保护]