MgSO_4对神经元NMDA兴奋毒性损伤的剂量依赖保护作用  

Protective effect of Mg^(2+) on primary cortical damage in rats caused by NMDA

作　　者：郁毅刚[1] 蔡颖谦[2] 王文浩[1] 林俊明[1] 黄巍[1] 罗飞[1] 李君[1] 胡连水[1] 

机构地区：[1]解放军第一七五医院神经外科,福建漳州363000 [2]南方医科大学珠江医院全军神经医学研究所神经科,广东广州510282

出　　处：《临床和实验医学杂志》2008年第10期6-7,9,共3页Journal of Clinical and Experimental Medicine

基　　金：军队十一五医学科研基金面上项目(06MB152);国家自然基金面上项目(30672167);福建省科技计划青年人才项目(2006F3150);福建省漳州市科技计划项目(Z06078)资助

摘　　要：目的研究镁离子(Mg2+)对神经细胞兴奋性氨基酸N-甲基-D-天冬氨酸(NMDA)损伤的保护作用。方法采用原代培养的大鼠皮层神经细胞模型,给予NMDA损伤,加入不同浓度的Mg2+,测定神经细胞损伤噻唑蓝(MTT)变化。结果Mg2+对兴奋性氨基酸损伤保护作用在1.0~3.0 mmol/L范围内存在,2.0 mmol/L效果最可靠。结论在重型颅脑损伤的临床治疗中应尽早使用镁剂,剂量根据脑脊液浓度尽快调整到2.0 mmol/L保护作用最好。Objective To investigate the neuroproteetive effect of Mg^2＋ for neurons damaged by NMDA. Methods By using primary cultured rat cortical neurons, the change in MTT of neurons damaged by NMDA and protective effect of Mg^2＋ were observed. Results The neuroprotective effect of Mg^2 ＋ related to the concentration of Mg^2＋ between 1.0 - 3.0 mmol/L and effective time. 2.0mmol/L is the best concentration for neuroprotective effect. Conclusion Mg^2＋ should be clinically applied as early as possible, and the dosage should be adjusted to 2.0 mmol/L based on its concentration in CSF.

关 键 词：NMDA受体 镁离子(Mg^2+) 脑损伤 

分 类 号：R96[医药卫生—药理学]