淡色库蚊对溴氰菊酯抗性的分子生物学检测  

Molecular biological monitoring of phyrethoid resistance in Culex pipiens pallens

作　　者：褚宏亮[1] 周明浩[1] 杨维芳[1] 张爱军[1] 

机构地区：[1]江苏省疾病预防控制中心,江苏南京210009

出　　处：《中华卫生杀虫药械》2008年第5期341-342,共2页Chinese Journal of Hygienic Insecticides and Equipments

基　　金：江苏省疾病预防控制中心青年科研基金项目(JSQ200503);江苏省预防医学科研课题指导项目(YZ2006010)

摘　　要：目的建立一种可以早期检测蚊虫对拟除虫菊酯抗药性的方法。方法采用聚合酶链式反应(PCR)方法,用特异性引物对蚊虫的钠离子通道基因进行扩增。结果敏感品系中只检测出SS(敏感纯合子)型,抗性品系中检测出SS型、RS(杂合子)型和RR(抗性纯合子)型。结论PCR能够快速完成蚊虫对拟除虫菊酯抗药性的检测,为蚊虫对拟除虫菊酯抗药性的早期监测提供了快速可行的方法。Objective To establish a method that could monitor phyrethoid-resistance of Culex pipiens pallens in early stage. Methods PCR method was applied to detect the sodium channel gene of Culex pipierts pallens with special primers. Results Only type SS was tested in susceptible strain of Culex pipiens pallens. Type SS, RS and RR were tested in resistant strain. Conclusion PCR can be applied to distinguish susceptible and resistant phyrethroid Culex pipiens pallens within short time. It is valuable in rapid detection of phyrethroid resistance of mosquitoes.

关 键 词：淡色库蚊 溴氰菊酯 抗性 聚合酶链式反应 

分 类 号：S481.4[农业科学—农药学]