大鼠血管性痴呆模型的改良  

Improvement of Vascular Dementia Model in Rats

作　　者：郑敏[1] 赵骥[1] 陈红光[1] 王帮华[2] 鲍翠玉[1] 

机构地区：[1]咸宁学院中心实验室 [2]咸宁学院基础医学示范中心

出　　处：《咸宁学院学报（医学版）》2008年第4期277-278,284,共3页Journal of Xianning Univarsity(medical Sciences)

基　　金：湖北省自然基金项目(2006ABA335);湖北省教育厅团队项目(T200606);湖北省教育厅优秀青年基金资助项目(Q200628006);咸宁学院优秀中青年科技创新团队项目(KT0735)

摘　　要：目的对大鼠血管性痴呆双侧颈总动脉永久性结扎模型(permanent bilateral common carotid artery oc-clusion,2VO)进行改良,提高模型动物存活率。方法采取改良造模方法(间隔3天分2次结扎双侧颈总动脉)和传统的2VO方法(同时结扎双侧颈总动脉)建立血管性痴呆模型,观察并比较2种方法大鼠的成活率和学习记忆能力差异。结果术后60d,改良模型组动物存活率(80.0%)明显高于传统模型组(45.0%)(P<0.01)。2个模型组大鼠学习记忆能力无显著性差异(P>0.05),而均显著低于假手术对照组(P<0.01)。结论改良2VO法是建造大鼠血管性痴呆模型的理想方法。Objective To establish a better permanent bilateral common carotid artery occlusion method which is used to establish the vascular dementia model and increase survival rate of the model animals.Methods The bilateral common carotid arteries were occluded in two times operation at 3 days interval for improved method or,blocked simultaneously at one surgery for traditional one.The differences between the two methods about the survival rates of the rats,and the learning and memory ability were compared.Results 60 days after the last operation,the survival rate of the improved model group animals（80%） was much higher than that of the traditional group animals（45%）（P〈0.01）.The learning and memory ability of both model groups showed no statistical difference（P〉0.05）,and was decreased as compared to the control group（P〈0.01）.Conclusion The improved permanent bilateral common carotid artery occlusion is a better method to establish the vascular dementia model than the traditional one.

关 键 词：动物模型 血管性痴呆 MORRIS水迷宫 大鼠 

分 类 号：R33-33[医药卫生—人体生理学]