白细胞介素-6在全身炎症反应中对冠脉内皮细胞的作用  

The role of IL-6 in cornary endthiline cell injury during systemic inflammatory response syndrome

作　　者：张红超[1] 于鲁峰[1] 李玲可[1] 杨玉红[1] Tao W.K Shi Chen 

机构地区：[1]空军总医院心外科,北京100036 [2]美国西南医学中心胸心外科麻醉专业组

出　　处：《中华实验外科杂志》2008年第10期1292-1293,共2页Chinese Journal of Experimental Surgery

摘　　要：目的应用基因敲除小鼠模型研究白细胞介素（IL）-6在这一损伤过程中对内皮细胞的损伤作用及其机制。方法IL-6knockout（IL-6KO，C57BL／6J-116tmlKopf，实验组）小鼠与其野生型小鼠C57BL／6J（对照组）分别给予40％烧伤，用Langendorff离体工作心模型观察烧伤后鼠心心功能及冠脉流量的变化，并观察内皮细胞对乙酰胆碱的反应性，用HE和Tunel染色观察心肌血管及其内皮细胞的变化。结果在非烧伤应急情况下，对照组与实验组的冠脉流量、心排出量及对乙酰胆碱刺激的反应均无明显差异，病理学差异无统计学意义。但在烧伤后，对照组冠脉流量（1．94±0．17比2．32±0．21ml／min，P〈0．05）与心爿E量（3．7±0．3）比（7．5±0．4）ml／min，P〈0．05）均显著减少，对乙酰胆碱刺激的反应显著低于实验组（2．34±0．23）比（2．88±0．26）；（5．0±0．4）比（8．4±0．4），P〈0．05）；病理学上对照组血管内皮肿胀、排列紊乱及内皮细胞凋亡现象。结论循环血液中IL-6对内皮细胞的损伤作用可能是其心功能抑制的始动环节。Objective By using the IL-6 deficiency mice,to study the role of IL-6 in comary endothelial cell （EC） injury during systemic inflammatory response syndrome （SIRS）. Methods The TBSA bum mice model was used to simulate the clinicopathological process. IL-6 knock out mice （ IL-6 KO, C57BL/6J-I16tmlKopf） were used for evaluating the role of IL-6 in this process. The wild type C56/BL6 male and IL-6 knock out mice were burned in 40% respectively. In addition to observation of cornary flow （CF） and cardiac function with Langendorff method in 24 h after burn, acetylcholine （ 10s mol/L, 10 ml/ 5 min） was administered to K-H perfusion solution to study the change of cornary flow （CF）. At the final, pathological morphologic changes were analyzed with H＆E and Tunel stain. Results Without burn, there were no difference between the two groups in cornary flow,cardiac output （CO） and acetylcholine stimula- tion as well as pathological changes. However, there was significant difference between the control group and knockout group in the CF （1.94±0.17 vs 2.32±0.21 ml/min,P 〈0.05） and CO （3.7±0.3 vs 7.5±0.4 ml/min, P 〈 0.05 ） after burn. There also was significant different response to acetylcholine （ CF：2.34 ± 0.23 vs 2.88±0.26 ; CO ：5.0±0.4 vs 8.4 ± 0.4,P 〈 0.05）. In IL-6 knock out mice there was milder EC injury and appotosis in heart pathology. Conclusion The cytokine IL-6 is a risk factor in bum pathogenesis. But IL-6 deficiency seems to be advantageous for cardiac EC injury suffering from burn.

关 键 词：心脏 内皮细胞 烧伤 乙酰胆碱 全身炎症反应 

分 类 号：R459.7[医药卫生—急诊医学] R543[医药卫生—治疗学]