低钾胁迫对苍术生长发育及挥发油的影响  

Effect of low-potassium stress on growth of Atractylodes lancea and components of its essential oil

作　　者：周洁[1] 郭兰萍[1] 黄璐琦[1] 张永清[2] 韩小丽[1,3] 

机构地区：[1]中国中医科学院中药研究所,北京100700 [2]山东中医药大学药学院,山东济南250014 [3]河南大学化学化工学院,河南开封475004

出　　处：《中草药》2008年第10期1548-1552,共5页Chinese Traditional and Herbal Drugs

基　　金：国家自然科学基金资助项目(30672616;30672617);国家科技支撑计划项目(2006BAI09B03);国家重点基础研究发展计划项目(2006CB504700)

摘　　要：目的探讨低钾胁迫对苍术生长发育及其挥发油成分的影响,揭示低钾环境与苍术道地性形成的关系。方法通过苍术植株不同程度低钾胁迫受控实验,分析测定其生物量及植株全钾、可溶性蛋白、总挥发油量的变化,并采用GC-MS分离鉴定挥发油中各组分,使用SPSS分析软件进行统计。结果除根长外,低钾胁迫组的株高、根茎粗、叶片数、分枝数、须根数、地上鲜质量与干质量、地下鲜质量与干质量等指标均较正常组显著降低(P<0.05);植株全钾与可溶性蛋白质的量随培养液供钾水平的提高而升高;低钾胁迫组挥发油组分显示出质量分数较大的组分数目显著增多、各组分的量趋于均衡、茅术醇与β-桉油醇2个主要成分的量显著下降(P<0.05)。结论适度低钾胁迫导致的苍术挥发油组分变化与道地药材苍术的挥发油特征相符,低钾胁迫应是苍术药材道地性形成的重要逆境因子之一。Objective To study the effect of low-potassium stress on growth of Atratylodes lancea and components in its essential oil and reveal the relationship between low-potassium stress and the formation of geoherbs of A. lancea. Methods With controlled experiment of A. lancea under different low- potassium stress, the biomass, total potassium of plant, dissoluble proteins, and the content of essential oil were measured, and the components in essential oil were isolated and determined by GC-MS. The data were analyzed by SPSS software. Results Compared with the control group, the indexes of plant height, rhizome circumference, lamina number, ramous number, fibrous root number, fresh weight and dry weight of aerial and underground parts in low-potassium stress group were all significantly decreased except root length （P 〈0.05）; Total potassium and dissoluble proteins of plant increased with the increasing of potassium level of culture fluid; The content of components yielding more percent increased markedly. The content of different components trended to balance, and among them two main components, hinesol and β-eudesmol decreased significantly （P〈0.05）. Conclusion The change of the components in its essential oil induced by moderating low-potassium stress is consistent with the naphtha composing characteristics of geoherbs of A. lancea. The low-potassium stress is an important adversity factor in the formation for geoherbs of A. lancea.

关 键 词：苍术 低钾 挥发油 

分 类 号：R282.2[医药卫生—中药学]