龙胆药材的HPLC指纹图谱研究  

HPLC Fingerprint of Radix Gentianae

作　　者：江蔚新[1] 钦浩[1] 何文顺[1] 

机构地区：[1]哈尔滨商业大学药学院,黑龙江哈尔滨150076

出　　处：《中草药》2008年第10期1563-1565,共3页Chinese Traditional and Herbal Drugs

基　　金：黑龙江省自然科学基金资助项目(120341)

摘　　要：目的建立不同产地龙胆药材的HPLC指纹图谱,为整体控制和评价龙胆药材的质量提供依据。方法采用HPLC测定10个不同产地的龙胆药材,制定指纹图谱。色谱条件:ODS柱,甲醇-水为流动相梯度洗脱,检测波长254 nm,体积流量1.0 mL/min,柱温25℃。结果建立了龙胆药材HPLC指纹图谱的共有模式,并对不同产地的药材进行了相似度评价。结论HPLC指纹图谱稳定性、重现性好,简便可行,适用于龙胆药材的标准化种植及质量控制。Objective To establish the HPLC fingerprint of methanol-resoluble component in Radix Gentianae collected from different habitats and accordingly offer the evidence for quality control and quality evaluation of Radix Gentianae. Methods Ten batches of Radix Gentianae were measured by the RPHPLC, and their fingerprints were obtained. Chromatographic condition： ODS-column, gradient elution with methanol-water, the detection wavelength was at 254 nm, flow rate was at 1.0 mL/min, and the column temperature was 25℃. Results The mutual mode of Radix Gentianae was set up and then the similarity was compared with software which was published by the Committee of Pharmacopeia of China. Conclusion According to the analysis of statistics, this method is steady, accurate with good repeatability, and can be used as standardization implantation and quality control of Radix Gentianae.

关 键 词：龙胆药材 HPLC 指纹图谱 质量控制 

分 类 号：R282.7[医药卫生—中药学]