PD-1在器官移植患者T细胞上的表达及其意义  

Expression of PD-1 on T cells in allo-recipients and its significance

作　　者：吴海竞[1] 张光波 胡玉敏 明志君[1] 张学光 

机构地区：[1]苏州大学医学部药学院 [2]生物技术研究所,江苏苏州215123

出　　处：《南京医科大学学报（自然科学版）》2008年第10期1250-1253,1257,共5页Journal of Nanjing Medical University(Natural Sciences)

基　　金：江苏省高校自然科学研究计划(03KJB3201120);苏州大学医学发展基金(EE132031);苏州大学在职获得博士学位人员基金资助项目

摘　　要：目的:检测共刺激分子PD-1在应用免疫抑制剂的器官移植患者外周血单个核细胞(PBMCs)和T细胞上PD-1的表达,并对其表达的意义以及抗PD-1单克隆抗体与免疫抑制剂的协同作用进行探讨。方法:采用流式细胞分析技术,应用单标记和双标记的方法,对应用免疫抑制剂的肝移植和肾移植患者的外周血单个核细胞和T细胞上PD-1的表达进行检测。以混合淋巴试验在体外模拟移植模型,应用MTT法,观察PD-1单抗与免疫抑制剂的协同作用。结果:PD-1在应用免疫抑制剂的器官移植患者PBMCs和T细胞上有特异性高表达,环孢素A(CsA)能诱导PD-1在PBMCs上的表达,且抗PD-1单克隆抗体与CsA联用具有协同作用。结论:PD-1可作为抗移植排斥作用的免疫干预新靶点。Objective：To detect the expression of PD-1 on allo-recipients＇ PBMCs and T ceils administrated with immunosuppres sant and investigate the significance of positive cooperativity of PD-1 mAb combined with CsA on T cells. Methods：Double and single labeled FCM was used to detect the expression of PD-1 on PBMCs and T cells of patients. The mixed lymphocytes assay （MLA） was used to establish a model of the transplant rejection in vitro. MTr was used to detect the cooperativity of PD-1 mAb and CsA. Results：Compared with normal person,PD-I was highly expressed on T cells in allo-recipients who administrated with immunosup- pressant. In mixed lymphocytes assay,the expression of PD-I seemed to be induced by CSA,and PD-1 mAb plus CsA displayed a positive cooperativity,which would owe to the low intensity of costimulatory signal. Conclusion：PD-I might be used as a new target of immune interference of allograft survival.

关 键 词：PD-1 T细胞 免疫抑制剂 器官移植 

分 类 号：R392.7[医药卫生—免疫学]