非淀粉多糖酶对奥尼罗非鱼蛋白酶、淀粉酶活性影响的研究  

Effects of Non-starch Polysaccharide on Activities of Protease and Amylase of Hybrid Tilapia(Oreochromis Niloticus×O.Aureus.)

作　　者：王纪亭[1] 万文菊[2] 吴淑勤[3] 石存斌[3] 计成[4] 

机构地区：[1]山东农业大学动物科技学院,泰安271000 [2]泰山医学院基础医学部,泰安271000 [3]中国水产科学研究院珠江水产研究所,广州510380 [4]中国农业大学动物科技学院,北京100193

出　　处：《动物营养学报》2008年第5期599-605,共7页CHINESE JOURNAL OF ANIMAL NUTRITION

基　　金：山东省中青年科学家科研奖励基金项目(2006BS06018)资助

摘　　要：本文从生长性能角度研究了非淀粉多糖酶对奥尼罗非鱼肝胰脏、肠道(前、中后肠)蛋白酶和淀粉酶的影响。试验选用体重约60g的奥尼罗非鱼(Oreochromis niloticus×O.aureus)560尾,随机分成4组,每组7个重复,每重复20尾鱼,分别饲喂添加了不同水平非淀粉多糖复合酶制剂(0、0.01%、0.02%、0.04%)的基础日粮,喂料后0、1、3、5h对各组进行组织采样,研究非淀粉多糖酶对罗非鱼肝胰脏、肠道(前、中后肠)蛋白酶和淀粉酶的影响。结果表明:(1)添加0.02%和0.04%非淀粉多糖酶显著提高了奥尼罗非鱼的特定生长率、显著降低了饵料系数(P<0.05);(2)蛋白酶活性分别按肝胰脏、胃、肠道依次升高;(3)添加0.04%非淀粉多糖酶组罗非鱼肝胰脏、胃蛋白酶活性最大,均显著高于对照组;(4)除喂后5h采样结果外,其它时间采样检测结果均以添加0.01%非淀粉多糖酶组中后肠蛋白酶活性最高,显著高于对照组(P<0.05);(5)适量添加非淀粉多糖酶对肝胰脏淀粉酶活性影响不大,高量添加非淀粉多糖酶(0.04%)对肝胰脏淀粉酶活性具有抑制作用,在一定程度上提高了前、中后肠淀粉酶活性。Hybrid tilapias （ Oreochromis Niloticus x O. Aureus. ） were employed to study the effects of non-starch polysaccharide （NSP） enzyme on the activities of protease and amylase of different digestive organs and different time of 0, 1, 3, 5 hours after feeding. Five hundred and sixty fish were randomly divided into 4 groups with 7 replicates and 20 fish in each replicate. Four groups were fed with basal diets added with 0, 0.01%, 0.02% and 0.04% NSP-enzyme respectively. The resuits showed that： （1） 0.02 % and 0.04 % supplement of NSP-enzyme in the hybrid tilapia diet had significantly improved the special growth rate and feed conversion rate of hybrid tilapia（P〈0.05） ;（2）The activity of protease was gradually increased according to the order of liver-pancreas, stomach and intestines; （3） The protease activity of liver-pancreas and stomach of 0.04% enzyme supplementation group was the highest and significantly higher than that of control group（P〈0.05） ;（4） Except for the sampling results at the point of 5 hours after feeding, all other sampling detection results showed that the protease activity of middle-rear intestine in 0.01% enzyme supplementation group was the highest and significantly higher than that of control group （P〈0.05） ;（5） The activity of pancreatic-liver amylase was affected by NSP-enzyme with high levels and not affected by low levels of NSP-enzyme, the amylase activity of front and middle-rear intestines was increased by supplement of NSP-enzyme in a certain extent.

关 键 词：非淀粉多糖酶 罗非鱼 蛋白酶 淀粉酶 

分 类 号：S816.7[农业科学—饲料科学] S965.125[农业科学—畜牧学]