多重耐药大肠埃希菌复方新诺明耐药相关基因研究  

Study on the Genes of SMZco Resistance in Multi-drug-resistant Strains of Escherichia coli

作　　者：孙海平[1] 林宁[1] 

机构地区：[1]淮安市第一人民医院,江苏淮安223300

出　　处：《现代检验医学杂志》2008年第5期42-43,共2页Journal of Modern Laboratory Medicine

摘　　要：目的了解多重耐药大肠埃希菌(ECO)对复方新诺明耐药机制。方法采用PCR检测20株多重耐药ECO菌复方新诺明耐药基因(sul1,dfrA1,dfrA5,dfrA12,dfrA17)。结果sul1,dfrA1,dfrA12,dfrA17基因阳性率分别为90%(18/20),10%(2/20),5%(1/20),75%(15/20),未检出dfrA5基因。共有18株检出sul和/或dfrA基因(90%)。结论本组多重耐药ECO菌复方新诺明耐药与携带sul1,dfrA基因相关,复方新诺明耐药表型与基因型相符。Objective：To investigate mechanisms of SMZco resistance in multi-drug-resistant strains of Escherlchia coil Methods The genes（sull,dfrAl,dfrA5,dfrA12 and dfrA17） of SMZco resistance were detected by polymerase chain reaction（PCR）.Results In 20 strains,sull,dfrAl,dfrA12,dfrA17-positive rate was 90% （18/20）,10%（2/20）,5%（1/20） and 75%（15/20） respectively. A total of 18 detection sul and /or dfrA gene（90%）. Conclusion The SMZco resistance mechanisms of the ECO are sull and dfrA related. The SMZco resistance phenotype and genotype consistent.

关 键 词：大肠埃希菌 复方新诺明 耐药 

分 类 号：R378.21[医药卫生—病原生物学] Q781[医药卫生—基础医学]