人内皮抑素基因对人脐静脉内皮细胞增殖的抑制作用  

The Inhibition Effect of Human Endostain Gene on HUVEC Cell Proliferation

作　　者：尹万昕[1] 李朝辉[2] 王丽娜[2] 

机构地区：[1]吉林大学第二医院 [2]吉林省长春八一医院,长春130061

出　　处：《中国现代医生》2008年第28期24-24,35,共2页China Modern Doctor

摘　　要：目的研究人内皮抑素基因对人脐静脉内皮细胞增殖能力的影响。方法RT-PCR获取人内皮抑素基因,通过酶切,测序鉴定获取的hES,MTT法检测该基因对人脐静脉内皮细胞(HUVEC)增殖的影响。结果获得的hES基因经测序证实成功克隆人内皮抑素基因;hES基因转染HUVEC细胞24h、4h和72h,细胞增殖率均较正常组明显降低(P<0.05),而脂质体对照与载体对照与对照组无明显变化。结论hES基因能够抑制HUVEC增殖。Objective To investigate the reproductive activity that human endostain gene affects on HUVEC cells. Methods Human endostain gene was gained by PT-PCR,and measured the effect of human gene on HUVEC cells proliferation. Results The human endostain gene gained successfully by RT-PCR and was indeficated by sequencing; after human endostain gene transfecfion 24 h, 48 h and 72 h, the cell reproduced ratio deceased obviously than normal group （P 〈 0.05）. but liposome group and vector group had no marked change. Conclusion Human endostain gene can inhibit HUVEC cells proliferation.

关 键 词：人内皮抑素基因 RT-PCR 细胞增殖 

分 类 号：R446.9[医药卫生—诊断学]