阿魏酸钠对一氧化碳供体硝普钠诱导的海马神经元凋亡的保护作用  

Protective effects of Sodium Ferulate (SF) on apoptosis of cultured hippocampal neurons induced by nitric oxide-donor SNP

作　　者：张海风[1] 臧明玺[1] 单杰[1] 安玉会[1] 李道明[2] 

机构地区：[1]郑州大学基础医学院生化教研室,河南郑州450001 [2]郑州大学基础医学院病理教研室,河南郑州450001

出　　处：《中国现代医学杂志》2008年第18期2601-2604,2608,共5页China Journal of Modern Medicine

基　　金：河南省医学科技攻关项目(No:200703002)

摘　　要：目的探讨阿魏酸钠(sodium ferulate,SF)对NO供体硝普钠(SNP)引起的大鼠海马神经元凋亡的影响。方法采用MTT比色分析测细胞存活率,Hoechst33258荧光染色及DNA琼脂糖凝胶电泳分析等方法检测凋亡。结果不同剂量SF(10～160μmol/mL)预处理海马神经元6h可剂量依赖地对抗SNP引起的神经元凋亡,提高神经元的存活率;减少SNP引起的核固缩、凝聚和碎裂现象;DNA凝胶电泳图谱未见典型的"梯子状"改变。结论SF对NO供体SNP诱导的海马神经元凋亡具有明显的保护作用。[Objective] To study the effects of Sodium Ferulate （SF） on apoptosis of cultured hippocampal neurons induced by nitric oxide-donor, Sodium Nitroprusside （SNP）. [Methods] Neuronal viability and morphology were tested by MTT assay and light microscope. Huoreseent staining with Hoehest 33258 and agarose gel electrophoresis was used to analyze apoptosis. [Results] Pretreatment with different concentrations of SF （10-160 μmol/mL） for 6 h increased the survival rate of neurons in a concentration-dependent fashion. SF prevented the neuronal nuclei from shrinkage, condensation and cleavage induced by SNP. It also blocked neuronal nuclear DNA fragmentation induced by SNP. [ Conclusion] These results indicated that SF can protect neurons from apoptosis induced by NO.

关 键 词：阿魏酸钠 硝普钠 海马神经元 凋亡 

分 类 号：R338.1[医药卫生—人体生理学]