鞘内注射布托啡诺对SNI模型大鼠脊髓背角NMDAR表达的影响  

Effects of intrathecal Butorphanol on rat's pain threshold and changes of neuronal NMDA receptor expression in rat spinal dorsal horn following spared nerve injury of sciatic nerve

作　　者：魏小洁[1] 郭曲练[1] 王懿春[1] 

机构地区：[1]中南大学湘雅医院

出　　处：《中国现代医学杂志》2008年第18期2616-2621,共6页China Journal of Modern Medicine

摘　　要：目的采用免疫组织化学方法,观察鞘内注射布托啡诺对SNI模型大鼠痛阈及脊髓背角NMDAR表达的影响。方法健康雄性SD大鼠20只,随机分为4组:假手术组(C组,n=5)、生理盐水组(NS组,n=5)、布托啡诺12μg组(B1组,n=5)、布托啡诺24μg组(B2组,n=5)。根据改良Yaksh法进行鞘内置管。置管5d后,C组不结扎神经,其他3组按Woolf等方法建立神经病理性疼痛模型(SNI),制模2d后,C组和NS组用25μL微量注射器鞘内注射20μL生理盐水,B1、B2组则分别用微量注射器鞘内注射10μL布托啡诺12和24μg,然后用10μL生理盐水冲管(共20μL)。在30min后,行疼痛行为学观察。注药后2h处死大鼠,用免疫组化法观察大鼠腰5节段水平脊髓背角NMDAR的表达。结果NMDAR1主要分布于细胞膜及胞浆。显微镜下及免疫组化观察,NS组大鼠脊髓背角NMDAR免疫阳性细胞数密度与C组比较明显增加;B1组和B2组的阳性细胞数量与NS组比较阳性细胞数量减少,并且两者之间差异也有显著性(P<0.05);NS组,B1组和B2组的阳性细胞光密度值较C组增高(P<0.05)。结论鞘内注射24μg布托啡诺可减轻SNI模型大鼠痛觉过敏,且呈剂量依赖性;SNI模型引起大鼠脊髓背角神经元NMDAR表达上调;鞘内注射布托啡诺可降低SNI大鼠脊髓背角神经元NMDAR表达上调。[Objective] To observe the effect of intrathecal Butorphanol on rat＇s pain threshold and changes of nervronal NMDA receptor expression in rat spinal dorsal horn following spared nerve injury of sciatic nerve by using immunohistochemistry method. [Methods] Twenty healthy male Sprague-Dawley rats （200-270 g） were randomly devided into four groups （n =5,in each group）： control group （group C）, normal saline group （group NS）, Butorphanol 12μg group （group B1）, Butorphanol 24 μg group （group B2）. A microapinal catheter was insterted intratheeally into the lumbar region （about 8 cm） according to the method of Yaksh. After 5 days, group C involved exposure of the sciatic nerve and its branches without any lesion, the other groups were made to the spared nerve injury model （SNI）. group C, group NS, groupB1 and group B2 were intrathecally injected 20 μL normal saline or 10 μl Butorphanol （12 μg and 24 μg） followed by 10 μL normal saline after 2 days post-surgery, respectively. 2 hours after intrathecally injection, all rats were killed, and the L5 spinal cord were dissected for determination of the expression of NMDA receptor using immunohistochemieal techniques. [Result] The number of immunoreaetive soma in the superficial laminae of the L5 spinal cord was significantly incressed significantly in group NS than that in group C （P 〈0.05）. The number of immunoreactive soma in group B1 and B2 increased significantly compared with that in group NS, and there was also difference between group B1 and B2 （P 〈0.05）. The immunoreaetive soma quantity density of NMDAR was higher in group NS than those in group C （P〈0.05）, but the quantity density in group Bland B2 were lower than that in the group NS, and between group B1 and B2 the quantity density was also different （P 〈0.05）. The immunoreactive soma optical density of NMDAR was higher in SNI model group than that in control group （P 〈0.05）. [Conclusions] Intrathecal Butorphanol （24 μg） can lessen hy

关 键 词：神经病理痛 布托啡诺 脊髓 鞘内 N-甲基-D-天冬氨酸受体 

分 类 号：R965.1[医药卫生—药理学]