灭菌预处理污泥及其滤液产氢发酵的比较研究  

Comparison research of sterilization pretreated sludge and their filtrate on hydrogen production by anaerobic fermentation

作　　者：周屹[1] 郭亮[1] 李小明[1] 谢波[1] 曾光明[1] 李宇亮[1] 杨麒[1] 刘精今[1] 

机构地区：[1]湖南大学环境科学与工程学院,长沙410082

出　　处：《环境工程学报》2008年第10期1332-1338,共7页Chinese Journal of Environmental Engineering

基　　金：国际科技合作重点项目(2004DFA06200);新世纪优秀人才支持计划项目(NCET-04-0770)

摘　　要：剩余污泥中的固态物质包含大量的蛋白质和多聚糖等有机质,一直被认为是一种具有潜力的生物制氢原料。灭菌预处理的剩余污泥会释放出大量的有机质,真空抽滤后去除掉污泥中固态物质得到滤液。将预处理污泥与滤液分别作为底物,接种产氢菌Pseudom onas sp. GZ1后产氢发酵。测定2种不同底物发酵反应过程中氢气的产量,以及底物的变化(SCOD、可溶性蛋白质、总糖、pH值)。实验结果表明,利用预处理后的污泥滤液作为底物能够有效提高氢气产量。滤液发酵的产氢量达到了4.44 mg H2/g COD,比预处理污泥直接发酵提高了近3.3倍。本实验证明污泥中的固态物质在发酵过程中能释放出更多的营养物质,但实际上并不能被产氢菌有效地利用产生更多的氢气,并对其原因进行了探讨。Waste biosolids collected from activated sludge is a biomass containing a vast amount of polysaccharides and proteins, and thus is regarded as a potential substrate for bio-hydrogen. The sludge would release lots of organic matters after sterilization pretreatment, then the filtrate was obtained from vacuum filtering the pretreated sludge through a filter. Both the pretreated sludge and their pretreated filtrate were used as the substrates for hydrogen production by Pseudomonas sp. GZ1 respectively. The hydrogen yield and the change of soluble COD,protein,carbohydrate and pH value during the fermentation processes were measured. The results showed that filtrate could produce more hydrogen than the whole biosolids. It could produce a hydrogen yield of 4.44 mg H2/g COD using filtrate as substrate, approximately 3.3 times higher than the pretreated sludge. It was demonstrated that the solids phase in sludge could release more nutrients during fermentation, but it was disadvantage for hydrogen production, and the possible reasons for the phenomenon were analyzed.

关 键 词：生物制氢 厌氧发酵 污泥滤液 

分 类 号：X703.1[环境科学与工程—环境工程]