松树脂溃疡病菌及其近缘种AFLP分析  

AFLP Analysis of Fusarium circinatum and Relative Species

作　　者：廖太林[1] 叶建仁[1] 陈建东[2] 

机构地区：[1]南京林业大学 [2]江苏出入境检验检疫局,南京210009

出　　处：《林业科学》2008年第9期82-86,共5页Scientia Silvae Sinicae

基　　金：国家质检总局科技资助项目(2004IK075)

摘　　要：利用AFLP分子标记技术对松树脂溃疡病菌及其近缘种进行DNA指纹分析,筛选出10对多态性较高的引物。上述引物在供试的9种17个菌株中共扩增出298条带,其中有多态性带283条,多态性位点百分率为94.97%。每对引物产生的多态性位点百分率在89.29%～100%之间,表明松树脂溃疡病菌及其近缘种间在DNA水平上存在广泛变异。根据AFLP标记结果计算松树脂溃疡病菌及其近缘种间的相似系数,对它们的亲缘关系进行定量描述。聚类分析结果表明,菌株间的聚类与生物学种分类的结果一致。分析引物E-AT/M-CAA绘制的镰刀菌AFLP指纹图谱遗传多样性,确定李瑟组镰刀菌各交配群的特异带、差异带,区分供试的7个交配群。AFLP fingerprinting analysis of Fusarium circinatum Nirenberg and O＇ Donnell and relative species was carried out. Ten primer-paris that could generated abundant polymorphism fragments were screened out. Total 298 nucleotide acid fragments were amplified with the primers from the template of the seventeen strains of Fusarium spp., DNA and among-them 283 fragments among were polymorphic. Percentage of polymorphism fragments produced by each pair of AFLP primer pair was 94.97% in average, and varied from 89.29% - 100%. All these data indicated that considerable genetic variation existed among F. circinatum and relative species at DNA level. Molecular genetic distances among Fusarium spp. were calculated, and the relationship among them was described quantitatively. Compared with biological species. The result of cluster analysis were basically similar to the phenotypic classification of species. Analysis of genetic diversity of E-AT/M-CAA AFLP fingerprinting of Fusarium spp. detormined, unique and different bands for each species and all Fusarium section Liseola tested were identified based on the E-AT/M-CAA AFLP fingerprinting.

关 键 词：松树脂溃疡病菌 AFLP 亲缘关系 鉴定 

分 类 号：S763.11[农业科学—森林保护学]