碳纳米管与PVA协同加强光谱探针并应用于灵敏测定肝素钠  

Enhancing Spectral Probe Based on the Synergic Effect of Carbon Nanotubes and PVA for the Sensitive Determination of Heparin

作　　者：官杰[1] 王宗花[1] 孙锡泉[1] 张菲菲[1] 夏延致[1] 

机构地区：[1]青岛大学国家培育基地-纤维新材料与现代纺织重点实验室,山东青岛266071

出　　处：《材料工程》2008年第10期257-260,264,共5页Journal of Materials Engineering

基　　金：国家自然科学基金资助项目(20475029);山东省优秀中青年科学家科研奖励基金资助项目(2004BS04008);国家基础研究项目(2006CB708603);山东省泰山学者奖励项目

摘　　要：提出将碳纳水箭（CNTs）作为一种新型分析增效试剂用于加强光谱探针，研究了其与水溶性大分子增效试剂聚乙烯醇（PVA）的协同增敏机理，并应用于乙基紫（EV）光度法测定肝素钠（Heparin．Hep）的体系中。在此缔合物显色体系中，含芳香环大分子的EV通过π-π作用于CNTs的管肇上，增大了行色物质的吸光面积；PVA的加入提高了CNTs及离子缔合物的分散性。结果表明，在CNTs与PVA复配的影响下，EV与Hep在pH为6．5的Britton—Robinson（B—R）缓冲溶液中形成稳定的紫红色缔合物后在610nm处发生明显的褪色，△Amax提高了330．7％。肝素钠浓度在0～2．5mg／L范围内与△A成正比．回归方程为△A=-0．006＋0．360C（mg／L），表观摩尔吸光系数ε=4．29×10^6L·mol-1·cm，R=0．9991。在最佳实验条件下，对肝素钠注射液效价进行了测定。The carbon nanotubes （CNTs） were regarded as a novel kind of enhancing agent to improve the effect of spectral probe, and a novel fading spectrophotometric method with the synergic effect of CNTs and PVA for the determination of Hep with Ethyl Violet （EV） is proposed in the present pa per. The mechanism of the synergic effect of CNTs and PVA was discussed in this work. The ion as- sociation complex of Hep and EV noncovalented with CNTs through π- stacking, the hydrophilic property of which was improved in the presence of PVA. Comparative experiments showed that △Amax of EV with Hep increased by 330.7% at 640nm in the presence of CNTs and PVA. The apparent molar absorptivity was 4.29 ×10^6 L. mol-1 ·cm-1. The linearity of the Hep in mass concentration ran- ges from 0-2.5mg/L of solution with a correlation coefficient of 0.9991. This method has been successfully applied to the determination of Hep in Heparin injection.

关 键 词：碳纳米管 乙基紫 光谱探针:肝素钠 协同加强 

分 类 号：O657.32[理学—分析化学]