大脑性分化前外源雄激素对雌鼠下丘脑性别二型神经元及前腹侧室旁核结构的影响  

Effect of exogenous androgen on structures of sexually dimorphism nucleus in preoptic area and anteroventral periventricular nucleus before sexual differentiation in female rats

作　　者：黄满丽[1] 魏宁[1] 胡健波[1] 许毅[1] 

机构地区：[1]浙江大学医学院附属第一医院,浙江杭州310003

出　　处：《浙江大学学报（医学版）》2008年第5期483-486,共4页Journal of Zhejiang University(Medical Sciences)

基　　金：浙江省自然科学基金项目(399139)

摘　　要：目的:观察大脑性分化前雄激素对下丘脑视前区性别二型神经元(SDN-POA)和前腹侧室旁核(AVPV)的影响。方法:12只新生雌鼠随机分成两组:雄激素处理组(雄激素组):在雌鼠出生后第1天腹腔内注射丙酸睾丸素50μl(TP,10.0g/L);对照组:用相同方式注射相同体积的无菌油50μl。60d后取鼠脑,经尼氏染色,LEICA Q Win系统标记SDN-POA和AVPV边界并计算其体积。结果:经雄激素处理的雌鼠组SDN-POA体积明显大于对照雌鼠组[(16.77±2.68)vs(8.99±1.42)mm3×10-3,P<0.01],而AVPV体积明显小于对照雌鼠组[(9.14±1.16)vs(14.62±2.80)mm3×10-3,P<0.01]。结论:在雌鼠大脑性分化前给予外源雄激素可导致下丘脑SDN-POA体积增大,而AVPV体积减小。Objective： To investigate the effects of androgen on sexually dimorphism nucleus in preoptic area （SDN-POA） and anteroventral periventricular nucleus （AVPV） before sexual differentiation of the brain in female rats. Methods： Neonatal female SD rats （n=12） were randomly divided into two groups：androgen group and control group. Twenty-four hours after birth animals were subjected to intraperitoneal injection of 50 μl of testosterone propionate （TP,10.0 g/L） or aseptic oil as control. The rats were sacrificed 60 days after the injection and the brains were collected for crystal violet staining. LEICA Q Win system was applied in detecting the boundaries of SDN-POA and AVPV,then the volumes of SDN-POA and AVPV were calculated. Results： The volumes of SDN-POA in androgen group were significantly larger than those in control group [（16.77±2.68） vs （8.99±1.42）mm^3×10^-3,P〈0.01],while the volumes of AVPV in androgen group were significantly smaller than those in control group [（9.14±1.16） vs （14.62±2.80）mm^3×10^-3,P〈0.01]. Conclusion： Exogenous androgen rendered before sexual differentiation in female rats results in enlargement of SDN-POA volumes and reduction of AVPV.

关 键 词：雄激素类/药理学 下丘脑 下丘脑室旁核 神经元 注射 腹腔内 脑/病理学 睾酮/药理学 

分 类 号：Q42[生物学—神经生物学]