TGF-β减轻异基因骨髓移植小鼠急性移植物抗宿主病  

Transforming Growth Factor β Alleviates Acute Graft-versus-Host-Disease after Allogeneic Bone Marrow Transplantation in Murine Model

作　　者：王旖旎[1] 冯翠翠[1] 刘锦丽[1] 李芳[1] 付丽[1] 王昭[1] 

机构地区：[1]首都医科大学附属北京友谊医院血液科,北京100050

出　　处：《中国实验血液学杂志》2008年第5期1135-1139,共5页Journal of Experimental Hematology

摘　　要：为了探讨转化生长因子β(TGF-β)对小鼠异基因骨髓移植后急性移植物抗宿主病发生发展的影响,用C57BL/6小鼠作为供鼠,BABL/c小鼠为受鼠,建立小鼠同种异基因骨髓移植模型。BABL/c受鼠随机分为4组:空白对照组、单纯照射组、移植对照组和TGF-β实验组。TGF-β实验组于移植前2天至移植后7天每日皮下注射TGF-β1(1μg/kg)。结果显示:TGF-β实验组小鼠生存时间明显长于移植对照组(p<0.01),TGF-β实验组小鼠小肠、皮肤及肝脏GVHD病理改变明显轻于移植对照组。移植后7天TGF-β实验组小鼠血清IL-2浓度较空白对照组高,但远低于移植对照组;TGF-β实验组血清IL-10浓度明显高于移植对照组。结论:TGF-β能够减轻或抑制小鼠allo-BMT后致死性的aGVHD发生,提高移植后存活率。TGF-β可能使Th1细胞向Th2细胞偏移,抑制IL-2等Th1类细胞因子的分泌,同时促进了Th2类细胞因子IL-10的表达,降低aGVHD的发生。This study was purposed to investigate the effects and mechanism of transforming growth factor beta （TGF-β） on acute graft-versus-host disease （aGVHD） after allogeneic bone marrow transplantation （allo-BMT）. The recipients were male BABL/c mice, while the donors were male C57BL/6 mice. The routine model of aGVHD had been established by allo-BMT with donor derived T cells. Experiment was divided into four groups： control group, radiation control group, transplantation control group and TGF-β treated group. Mice in TGF-β treated group were daily subcutaneaesly injected TGF-β1 （1 μg/kg） in two days before transplantation until seven days after it. The results showed that the survival time of mice in TGF-β treated group was significantly longer than that in transplantation control group, and the aGVHD pathological changes in TGF-β treated group were milder than that in transplantation control group. At seven days after transplantation, the level of IL-2 in TGF-β treated group was significantly higher than that in control group, but significantly lower than that in transplantation control group. The level of IL-10 in TGF-β treated group was significantly higher than that in transplantation control group, but the level of IL-10 in transplantation control group was significantly lower than that in other groups. It is concluded that TGF-β may alleviate or suppress lethal aGVHD, and elevate the survival rate after allo-BMT in murine model. Accommodatin of the Thl and Th2 cytokine levels is the possible mechanism of TGF-β preventing lethal aGVHD.

关 键 词：转化生长因子Β 异基因骨髓移植 急性移植物抗宿主病 细胞因子 

分 类 号：R547.7[医药卫生—心血管疾病]