高浓度葡萄糖诱导红细胞磷脂酰丝氨酸暴露的机制研究  

Mechanism of Erythrocyte Phosphatidylserine Exposure Induced by High Concentrated Glucose

作　　者：权国波[1] 韩颖[1] 杨超[1] 扈文博[1] 刘敏霞 刘安[1] 王艳[1] 王捷熙[1] 

机构地区：[1]军事医学科学院野战输血研究所,北京100850

出　　处：《中国实验血液学杂志》2008年第5期1181-1184,共4页Journal of Experimental Hematology

基　　金：国家自然科学基金项目;编号30400518;北京市自然科学基金;编号7072059;军队"十一五"青年学者项目;编号06Q90

摘　　要：本研究探讨高浓度葡萄糖诱导红细胞磷脂酰丝氨酸(PS)暴露的机制。将红细胞于葡萄糖缓冲液中孵育后用流式细胞术分析PS标记率和前向角值;检测caspase-3和caspase-8的活化情况;用流式细胞术和荧光显微镜观察亮抑酶肽对葡萄糖引起的红细胞PS暴露的抑制效果。结果表明:葡萄糖可以诱导红细胞PS暴露,而且其效率和葡萄糖浓度密切相关。随着葡萄糖浓度的增加,红细胞的PS标记率呈逐步增加的趋势,当葡萄糖浓度为0.8mol/L时,红细胞的PS标记率在80%以上。然而,在葡萄糖引起的红细胞PS暴露过程中,caspase-3和caspase-8没有活化,而亮抑酶肽却可以很好地抑制红细胞PS暴露和使其体积缩小。随着亮抑酶肽浓度的增加,红细胞PS标记率呈逐步下降的趋势,而细胞体积却呈逐步增加的趋势。结论:高浓度葡萄糖可以导致严重的红细胞PS暴露,而且这种PS暴露和caspase无关,推测葡萄糖诱导的红细胞PS暴露是受一条对亮抑酶肽高度敏感的、未知的通路调控的。This study was aimed to investigate the induced by high concentrated glucose. After exposure mechanism of phosphatidylserine exposure of human erythrocytes to high concentrated glucose, the phosphatidylserine （PS） exposure and forward scatter value were analyzed by flow cytometry; the activities of caspase-3 and caspase-8 were detected; The inhibitory effect of leupeptin on cell PS exposure induced by high concentrated glucose was observed by flow cytometry and fluorescent microscopy. The results showed that the high concentrated glucose could induce PS exposure of erythrocytes and this inducing efficiency was dependent on the glucose concentrations. With increase of the glucose concentrations, the percentages of cells with exposed PS also increased. When the glucose concentration was 0.8 mol/L, the PS exposure was over 80%. However, caspase-3 and caspase-8 were not activated during PS exposure of cells induced by high concentrated glucose, but leupeptin could significantly inhibit PS exposure and volume shrinkage induced by high concentrated glucose. With increase of the leupeptin concentrations, the percentage of cells with exposed PS decreased and the cell volume increased. It is concluded that the high concentrated glucose can result in serious PS exposure, which does not depend on caspase. It can be hypothesized that the PS exposure of erythrocytes induced by high concentrated glucose may be controlled by an unknown pathway sensitive to leupeptin.

关 键 词：红细胞 磷脂酰丝氨酸 葡萄糖 亮抑酶肽 

分 类 号：R331.141[医药卫生—人体生理学]