血小板保存3天后再冷冻保存的实验研究及临床应用  

Cryopreservation of Platelets after Storage at Normal Temperature for 3 Days and Its Clinical Practice

作　　者：丁国良 

机构地区：[1]山东省东营市中心血站,山东东营257091

出　　处：《中国实验血液学杂志》2008年第5期1196-1200,共5页Journal of Experimental Hematology

摘　　要：本研究探讨血小板常温保存3天后再进行-80℃冰箱内冷冻保存及临床应用的可行性。对当天冷冻和保存3天后再冷冻血小板进行了计数,并检测聚集力、黏附力以及CD62p的表达,并通过可对比性病例观察保存3天后再冷冻与当天冷冻血小板临床应用的可能性。结果表明:在保存期3天之内血小板数量、低渗性休克反应、黏附功能无显著差异性改变(p>0.05),但聚集功能和CD62p的表达率的变化有显著性差异(p<0.05)。当天保存并冷冻的血小板与保存3天后再冷冻的血小板各项指标的变化都无显著性差异。CD62p的再表达率(CD62p凝血酶激活后阳性率-CD62p激活前的阳性率)也无显著性差异,分别是51.1±4.5和51.1±4.4(p>0.05)。临床应用当天冷冻和保存3天后再冷冻血小板的CC I值分别是48%和49%,无显著性差异(p>0.05)。结论:血小板保存3天后可以再-80℃冷冻保存,其临床应用效果与当天冷冻血小板比较后无明显差异。This study was purposed to investigate the feasibility of cryopreservation platelets under -80℃ after stored for 3 days at normal temperature and its clinical use. The platelet count, aggregation functions, adhesive ability, hypotonic shock reaction and CD62p expression of platelets preserved at normal temperature, cryopreserved on same day of collection and after storage for 3 days were detected, and possibility of using platelets cryopreserved after storage for 3 days in clinic was evaluated by comparison of contractable clinical cases. The results indicated that no signifteant differences in platelet count, hypotonic slock reaction and adhesive ability were found within 3 days of storage （p 〉 0. 05）; but differences in aggregation function and CD62p expression were significant （p 〈0.05）. As compared with platelets cryopreserved on same day of collection, the detected parameters such as platelet count, aggregation function, adhesive ability, hypotonic shock reaction, expression at storage for 3 days did not show significant differences （p 〉 0. 015 ; CCI value in clinically use of them also did not show significant difference too （p 〉 0.05 ）. It is concluded that the platelets after storage for 3 days can be cryopreserved, the efficiency of them in clinical use is not significant difference from platelets cryopreserved on same day of collection.

关 键 词：血小板 血小板保存 冷冻血小板 

分 类 号：R331.143[医药卫生—人体生理学]