阿尔茨海默病中mir-222作用机制的初步研究  

The Role of mir-222 in Alzheimer's Disease

作　　者：王晓映[1] 宗园媛[1] 张连峰[1] 秦川[1] 

机构地区：[1]中国医学科学院实验动物研究所,北京协和医学院比较医学中心,北京100021

出　　处：《中国比较医学杂志》2008年第9期13-15,I0003,共4页Chinese Journal of Comparative Medicine

摘　　要：目的探讨mir-222在阿尔茨海默病发病机制中的作用。方法取6月龄APPswe/PSΔE9小鼠脑组织,进行microRNA芯片的检测;利用real-time PCR对芯片检测结果进行验证;构建mir-222表达载体,将其转染至SH-SY5Y细胞中,转染后48小时提取蛋白检测p27kip1的表达情况。结果芯片结果显示,与野生型C57BL/6J小鼠相比,6月龄APPswe/PSΔE9小鼠脑组织中mir-222表达明显下降,经real-time PCR验证,差别具有统计学意义(P=0.012);将mir-222表达载体转染至SH-SY5Y细胞后,p27kip1表达减少。结论mir-222可能通过调节细胞周期抑制因子p27kip1的表达参与阿尔茨海默病的发生。Objective To explore the role of mir-222 in Alzheimer＇s disease. Methods a microRNA array was used for the analysis of microRNA expression from brain tissue of 6 months old APPswe/PS△E9 mouse. The results of microRNA array was validated by real time PCR. We constructed the mir-222 expression vector and transfected it into SH-SYSY cell line and total protein was extracted from the cells 48 hours after transfection. Results the data of microRNA array and real time PCR showed reduced compared with age-matched controls, the expression the expression level of p27kipl was detected by western blot. that the expression level of mir-222 in APPswe/PS△E9 mouse was level of p27kipl in SH-SYSY cells transfected with mir-222 expression vector was reduced compared with that transfected with negative control plasmids. Conclusion mir-222 may play a role in pathogenesis of Alzheimer＇ s disease by affecting the expression of p27kipl.

关 键 词：阿尔茨海默病 MICRORNA 转基因小鼠 microRNA芯片 

分 类 号：R541[医药卫生—心血管疾病]