甲氨喋呤对胶原诱导性关节炎大鼠滑膜骨桥蛋白和整合素α_Vβ3表达的影响  

Effect of Methotrexate on Expression of Osteopontin and Integrinαvβ3 in Synovium of CIA Rats

作　　者：柯丹[1] 孔芳[1] 胡绍先[1] 秦瑞芳[1] 苏敏[1] 沈桂芬[1] 涂巍[1] 

机构地区：[1]华中科技大学同济医学院附属同济医院风湿免疫科,武汉市430030

出　　处：《医学分子生物学杂志》2008年第5期408-411,共4页Journal of Medical Molecular Biology

基　　金：湖北省自然科学基金(No.2007ABA110)~~

摘　　要：目的探讨甲氨喋呤对胶原诱导性关节炎(collagen-induced arthritis,CIA)大鼠滑膜骨桥蛋白(osteopontin,OPN)及其受体整合素αvβ3表达的影响及其作用机制。方法建立CIA大鼠模型,分为模型组和甲氨喋呤(MTX)组,后者用MTX进行干预治疗;采用免疫组织化学染色技术检测滑膜组织OPN和整合素αvβ3的表达,ELISA方法检测血清TNF-α水平。结果①CIA模型组和MTX组大鼠的滑膜OPN、整合素αvβ3表达均明显高于正常大鼠对照组(均为P<0.01);与模型组比较,MTX组OPN和整合素αVβ3的表达减少(均为P<0.01)。②正常对照组和MTX组大鼠血清TNF-α水平显著性低于模型组大鼠(均为P<0.01)。结论滑膜OPN和整合素αvβ3异常表达在CIA发病中具有重要意义。MTX通过下调滑膜OPN和整合素αvβ3的表达,降低血清TNF-α水平从而达到治疗CIA的作用。Objective To investigate the effect of methotrexate （MTX） on expression of osteopontin and integrin αvβ3 in synovium of collage-induced arthritis （CIA） rats. Methods To establish CIA rat model, rats were divided into CIA model group and MTX treated group. Immunohistochemistry assay was used to detect the expression of OPN and integrin αvβ3 in articular synovium. TNF-α level in serum was detected by ELISA. Results （1） The expression of OPN and integrin αvβ3 in synovium of CIA model group and MTX treated group was significantly higher than that in control group （all P 〈0. 01 ） ; nevertheless, the expression level in MTX treated group was lower than CIA model group （ P 〈 0. 01 ） . （2） The level of TNF-α in both control group and MTX treated group was significantly decreased, compared with CIA model group （ all P 〈 0. 01 ） . Conclusions The abnormal expression of OPN and integrin αvβ3 in synovium might play an important role in the pathogenesis of CIA. MTX treatment can down-regulate the expression of OPN and integrinαvβ3 in synovium, reduce TNF-α level in serum, and inhibit progress of CIA.

关 键 词：关节炎 实验性 甲氨喋呤 骨桥蛋白 整合素ΑVΒ3 

分 类 号：R684.3[医药卫生—骨科学]