维拉帕米和生脉注射液对大鼠肝脏缺血再灌注损伤的保护作用  

THE PROTECTIVE EFFECT OF VERAPAMIL AND SHENGMAI INJECTION ON ISCHEMIA-REPERFUSION LIVER INJURY IN RATS

作　　者：山峰[1] 何俊峰[1] 宋暖[1] 刘淑红[1] 李堃[1] 王静[2] 王秋波[2] 

机构地区：[1]青岛大学医学院附属医院ICU,山东青岛266003 [2]青岛大学医学院分子与细胞免医学教研室

出　　处：《青岛大学医学院学报》2008年第5期390-392,共3页Acta Academiae Medicinae Qingdao Universitatis

基　　金：山东省卫生厅青年基金项目(2001CA2CKA1)

摘　　要：目的观察维拉帕米和生脉注射液对大鼠肝脏缺血再灌注损伤的保护作用。方法将40只大鼠分为假手术组（A组）、缺血再灌注组（B组）、维拉帕米组（c组）和生脉注射液组（D组），每组10只。A组开腹后再关腹；B组开腹后夹闭左肝蒂30min，再关腹；C组在开腹前静脉注射维拉帕米，开腹夹闭左肝蒂30min，再关腹；D组在开腹前3d每天经腹腔注射生脉注射液，开腹夹闭左肝蒂30min，再关腹。各组均在关腹48h后再开腹，每只大鼠取缺血肝组织检测查丙二醛（MDA）、热休克蛋白70（HSP70）的水平，光镜下观察肝组织的形态变化；取血检测谷丙转氨酶（ALT）、天门冬氨酸转氨酶（AST）、肿瘤坏死因子（TNF-α）的水平。结果B、C、D组ALT、AST、TNF-α及MDA测定结果与A组比较均有显著性差异（F=5.10-53.01，q=1.38-17.58，P〈0.05）；C、D两组ALT、AST、TNF-α及MDA含量均低于B组，差异具有显著性（q=2.89-10.08，P〈0．05），而C、D两组以上各指标除ALT比较差异有显著性（q=2.97，P〈0．05）外，其他指标比较差异无显著性（P〉0.05）。C、D两组HSP70的表达明显少于B组，而C组HSP70的表达略少于D组；C、D两组肝细胞肿胀、坏死程度均较B组轻。结论维拉帕米和生脉注射液可明显改善大鼠肝脏缺血再灌注后肝组织的损伤程度。Objective To observe the protective effect of Verapamil and Shengmai injection on ischemia-reperfusion injury in rat liver. Methods Forty healthy female Wistar rats were evenly randomized to sham operation group （group A）, the abdomen was opened and then closed; ischemia-reperfusion group （group B）, the blood supply of left hepatic lobe （LHL） was clamped for 30 minutes, the abdomen was then closed; Verapamil group （group C）, intravenous Verapamil was given before opening abdomen, the blood supply of LHL was blocked as in group B, the abdomen closed; and Shengmai injection group （group D）, Shengmai injection was administered intraperitoneally three days before the experiment. The ischemia of the LHL was done same as in groups B and C. The liver tissue was collected at 48 hours after the procedures and sent for detection of heat shock protein 70 （HSP70）, Malondialdehyde （MDA）. Serum tumor necrosis factor alpha （TNF-α）, alanine aminotrasferase （ALT）, and aspartate aminotrasferase （AST） were measured. Results Compared with group A, the levels of ALT, AST, TNF-α and MDA in groups B, C and D were significantly different （F=5.10-53.01,q=1.38-17.58,P〈0.05）. The ALT, AST, TNF-a and MDA in groups C and D were significantly decreased than those in group B （q= 2.89-10.08, P〈0.05）. Except of the significant difference in ALT between groups C and D （q=2.97 ,P〈0.05）, no significant differences of other parameters were found between this two groups. The expression of HSP70 in groups C and D was obviously lower than that in group B. While the HSP70 in group C was slightly decreased than that in group D. Compared with group B, the liver cellular edema and necrosis in groups C and D were effectively alleviated. Conclusion Verapamil and Shengmai injection can improve ischemia-reperfusion damage of rat liver.

关 键 词：维拉帕米 生脉注射液 肝脏 再灌注损伤 

分 类 号：R96[医药卫生—药理学] R285.5[医药卫生—药学]