抗hDR5单抗对食管癌细胞的凋亡及CDC作用观察  

Study on effects of anti-DR5 monoclonal antibody on apoptosis and CDC effect in EC109 cells

作　　者：黄红莹[1] 杜耀武[1] 白慧玲[1] 李淑莲[1] 马远方[1] 

机构地区：[1]河南大学免疫学研究所河南大学医学院河南大学天然药物与免疫工程省级重点实验室,开封475004

出　　处：《中国免疫学杂志》2008年第10期883-885,共3页Chinese Journal of Immunology

基　　金：国家自然科学基金(No30571697)项目

摘　　要：目的:观察抗hDR5单抗对食管癌细胞的凋亡作用及CDC作用。方法:采用杂交瘤技术制备单克隆抗体,采用MTT法及显微摄影观察McAb及补体作用后EC109的增殖及形态变化,流式细胞仪观察细胞凋亡。结果:获得了单克隆抗体,其对EC109的凋亡诱导作用呈剂量依赖性;与对照组比较,补体可显著提高单抗对EC109的细胞毒作用,二者联用其细胞生长抑制率可达83.04%,形态学观察可见凋亡小体及细胞溶解;结论:DR5单抗具有诱导EC109细胞凋亡的作用,与补体联合应用具有较强的CDC效应。Objective：To observe anti-DR5 monoclonal antibody on apoptosis and CDC effect in EC109 cells.Methods：The anti-DR5 monoclonal antibody was prepared by hybridoma technique.Tumoricidal effects and complement-dependent cytotoxicity of the McAb to EC109 cells was screened by MTT assay.The apoptosis of EC109 cells was detected by flow cytometry with annexin Ⅴ-FITC/PI staining.Morphological change of EC109 was observed by microphotograph.Results：An anti-DR5 monoclonal antibody was obtained.It induced apoptosis of EC109 dose dependently.The cytotoxic action was notably enhanced by addition of complement.The cells growth inhibition ratios reached 83.04%.The apoptotic body and cathepsis were seen in microphotograph.Conclusion：The anti-DR5 monoclonal antibody could induce EC109 cells apoptosis and cause the complement dependent cytotoxic （CDC） effects powerfully.

关 键 词：DR5 单克隆抗体 细胞凋亡 CDC效应 

分 类 号：R392-12[医药卫生—免疫学]