催乳素刺激Jurkat细胞热休克蛋白90的表达及地塞米松的干预作用  

Effect of dexamethasone on expression of HSP90 induced by prolactin in Jurkat cells

作　　者：许东明[1] 林玲[1] 黄子扬[1] 林夏鸿[1] 陈真[1] 

机构地区：[1]福建医科大学附属第二医院免疫内科,泉州362000

出　　处：《中国免疫学杂志》2008年第10期917-921,共5页Chinese Journal of Immunology

基　　金：福建省教委基金资助项目(JA04204);福建医科大学发展基金(FJGXY04001)资助

摘　　要：目的：探讨催乳素刺激下Jurkat细胞的热休克蛋白（HSP90）的表达状况以及地塞米松对这种表达的影响。方法：以四组不同浓度催乳素（25、50、100、200ng/ml）刺激Jurkat细胞,采用RT-PCR和Western blot检测刺激前后HSP90的表达水平;另将四种不同浓度的地塞米松（10^-9-10^-6mol/L）和高浓度催乳素（100ng/ml）与Jurkat细胞共同孵育,同法检测地塞米松干预后HSP90的表达水平。结果：所有浓度催乳素刺激Jurkat细胞在mRNA和蛋白质水平表达的HSP90均显著高于空白对照组（P〈0.05）,且表达水平随刺激浓度增加而升高,但当浓度高于100ng/ml以上时升高不明显。加入不同浓度地塞米松和高浓度催乳素孵育的Jurkat细胞表达HSP90水平与对照组比较有显著下降（P〈0.05）,且随地塞米松浓度升高而降低。结论：催乳素可刺激Jurkat细胞HSP90的表达,而地塞米松可抑制此效应且有剂量依赖关系。Objective：To investigate the effect of dexamethasone expressing level of HSP90 induced by prolactin in Jurkat cells.Methods：The expression of HSP90 in Jurkat cells was analyzed at mRNA and protein levels by either revese-transcription polymerase chain reaction（RT-PCR） or Westetn blot assays following incubation with each of four concentrations（25 ng/ml,50 ng/ml,100 ng/ml and 200 ng/ml）of prolactin.After cultured with dexamethasone at different concentrations（10^-9-10^-6 mol/L）,Jurkat cells were incubated with prolatin（final concentration of 100 ng/ml）,and the expression of HSP90 was analyzed.Results：All the groups cocultured with prolactin at different concentrations were shown higher levels of HSP90（P〈0.05）compared with the group in the absence of prolactin.The levels of HSP90 were depended on the concentrations of prolactin untill it reached 100 ng/ml.The groups cultured with both dexamethasone and prolactin were shown lower levels of HSP90（P〈0.05） compared with the blank control group.Conclusion：Prolactin can increase the expression of HSP90 in human leukemic T cell line（Jurkat cells）,and the maximal effection was shown on the concentration above 100 ng/ml of rhPRL.Dexamethasone can inhibit the expression of HSP90 in Jurkat cells stimulated by rhPRL.

关 键 词：催乳素 Jurkat细胞系 热休克蛋白90 

分 类 号：R392.6[医药卫生—免疫学]