雾化吸入布地奈德对肺纤维化大鼠肺组织羟脯氨酸和Ⅰ、Ⅲ型胶原mRNA表达的影响  

The effect of inhaled budesonide on the expression of hydroxyproline and type Ⅰ and Ⅲ collagen mRNA in lung tissue of bleomycin-induced rat pulmonary fibrosis.

作　　者：曾鸣[1] 刘丹[2] 王文军[1] 朱晨[1] 范贤明[1] 湛晓勤[1] 

机构地区：[1]泸州医学院附属医院呼吸内科,四川泸州646000 [2]重庆市第二人民医院放射科,重庆永川402160

出　　处：《四川医学》2008年第10期1293-1295,共3页Sichuan Medical Journal

基　　金：国家自然科学基金资助项目(30570814);四川省教育厅资助项目(05011);泸州医学院附属医院资助项目(06059)

摘　　要：目的探讨布地奈德（budesonide）雾化吸入对博莱霉素（bleomyein，BLM）致肺纤维化大鼠肺组织羟脯氨酸和Ⅰ、Ⅲ型胶原mRNA表达的影响。方法取健康雌性Wistar大鼠45只，随机分成博莱霉素组（BLM组）、生理盐水（NS组）和布地奈德组（Bud组）各15只，气管内灌注BLM（Bud组、BLM组）和生理盐水（NS组）后0～6d每天雾化1次，Bud组雾化吸入布地奈德，BLM组和NS组雾化吸入生理盐水，各组分别于第7、14、28d处死大鼠各5只。用HE、Masson染色观察肺泡炎症和纤维化改变；肺组织匀浆测定羟脯氨酸含量；逆转录-聚合酶链反应（reverse transcription-polymerase chain reaction，RT-PCR）测定肺组织Ⅰ、Ⅲ型胶原mRNA表达。结果Bud组第7、14d肺泡炎程度较BLM组减轻（P〈0．05），Bud组第7、14、28d肺纤维化程度均较BLM组减轻（P〈0．05）；Bud组第7、14、28d肺组织羟脯氨酸含量较BLM组明显降低（P〈0．05）；Bud组第7、14d肺组织Ⅰ、Ⅲ型胶原mRNA表达较BLM组明显降低（P〈0．05）。结论布地奈德雾化吸入可减轻BLM致肺纤维化大鼠肺泡炎症、降低肺组织羟脯氨酸含量和Ⅰ、Ⅲ型胶原mRNA表达，从而减轻纤维化程度。Objective To investigate the effect of inhaled budesonide on the expression of hydroxyproline and type Ⅰ /Ⅲ collagen mRNA in lung tissue of bleomyein-induced rat pulmonary fibrosis. Methods Forty-five adult female Wistar rats were randomly divided into 3 groups：both budesonide（Bud） group and bleomycin（BLM） group were intratraeheally instilled with BLM, and normal solution（ NS） group with 0.9% NaCl solution. Both NS group and BLM group were aerosolized with NS,while Bud group with budesonide ev ery day at day 0 - 6 after rats were intratracheally administrated. Each group was again divided into 3 subgroups,which were sacrificed at day 7,14 and 28. Hydroxyproline content was detected;HE and Masson staining were performed to observe the extent of alveolitis and fibrosis;The mRNA levels of type Ⅰ /Ⅲ collagen in the lung tissue were measured by RT-PCR.Results The extent of alveolitis in Bud group at day 7 and 14 were lower than those of BLM group（ P 〈 0.05 ）. The extent of lung fibrosis and the expressions of hydroxyproline in Bud group at day 7,14 and 28 were significantly lower than those of BLM group （ P 〈 0.05 ） ; The mRNA levels of type Ⅰ/Ⅲ collagen at day 7 and 14 in Bud group were significantly lower than those in BLM group（ P 〈 0.05 ）. Conclusion Inhaled budesonide could decrease the levels of hydroxyprotine and type Ⅰ /Ⅲ collagen mRNA and ameliorate the formation of pulmonary fibrosis in bleomycin-indueed rat pulmonary fibrosis.

关 键 词：布地奈德 肺纤维化 雾化吸入 

分 类 号：R563.9[医药卫生—呼吸系统]