何首乌的HPLC指纹图谱  

Fingerprint chromatogram of Radix Polygoni Multiflori by HPLC

作　　者：房志坚[1] 周洪波[1] 杨立伟[2] 陈浩桉[2] 

机构地区：[1]广东药学院中药学院,广东广州510006 [2]广东省药品检验所,广东广州510180

出　　处：《华西药学杂志》2008年第5期513-515,共3页West China Journal of Pharmaceutical Sciences

基　　金：广州市科技攻关重点项目(2007Z2-E5061);广东省科技计划项目(2007B031407003)

摘　　要：目的研究何首乌药材的指纹图谱。方法采用RP-HPLC法,以2,3,5,4′-四羟基二苯乙烯-2-O-β-D-葡萄糖苷为参照物,采用Zorbax SB C18色谱柱(250 mm×4.6 mm,5μm),流动相为甲醇-水,梯度洗脱,流速1 ml.min-1,检测波长254 nm。结果获得了较理想的指纹图谱,确定了9个共有峰,建立了何首乌共有模式色谱图,不同产地何首乌的相似度均大于0.9。结论所建方法简便、准确,重复性好,为何首乌的质量控制提供了依据。OBJECTIVE To establish HPLC chromatographic fingerprint of Radix Polygoni Muhiflori. METHODS 2,3,5,4＇ -tet- rahydroxystilbene - 2 - O -β - D - glucoside was emploged as standard substance for RP - HPLC methods. The seperation was per- formed on Zorbax SB - C18 column（250 mm ×4.6mm,5μm） ,with methanol and water as mobile phase for gradient elution. Flow rate was 1 ml· min-1 and the detection wavelength was 254 nm. RESULTS A satisfactory method for HPLC fingerprint determination of Radix Polygoni Multiflori from different sources was obtained, and 9 peaks on the chromatographic fingerprint of Radix Polygoni Mtdtiflori was determined, the mutual model of Radix Polygoni Multiflori was established and the similarities were calculated. The results showed the similarities were more than 0.9 in Radix Polygoni Muhiflori from different sources except 3 batches. CONCLUSION The method is simple and accurate with a good reproducibility and can be used as a standard method in controlling the quality of Radix Polygoni Multiflori.

关 键 词：何首乌 HPLC 指纹图谱 

分 类 号：R917[医药卫生—药物分析学]