薄层扫描法测定不同产地喜树果中的喜树碱  

Determination of camptothecin in Camptotheca acuminata fruit from different habitats by TLCS

作　　者：周芳礼[1] 黄瑞松[1] 苏青[2] 吴毅 陆珍[4] 林翠梧[1] 

机构地区：[1]广西大学化学化工学院,广西南宁530004 [2]广西民族医药研究所,广西南宁530001 [3]广西南宁食品药品检验所,广西南宁530001 [4]广西中医学院,广西南宁530001

出　　处：《华西药学杂志》2008年第5期610-612,共3页West China Journal of Pharmaceutical Sciences

基　　金：广西自然科学基金项目(桂科自0542055);广西大型仪器协作共用网资助项目(327-2006-016)

摘　　要：目的采用薄层扫描法测定喜树果中的喜树碱，评价不同产地喜树果药材的质量。方法采用羧甲基纤维素钠作为黏合剂；展开剂为氯仿-丙酮（7：3）；单波长线性扫描法测定，λs=360nm。结果喜树碱0．0542—0．3252μg与峰面积线的性关系良好，回归方程为：Y=97．19X-55．07（r=0．9928），平均回收率为97．13％，RSD=1．76％（n=6）。结论所建方法准确可靠，不同产地喜树果药材中喜树碱含量有一定差异。OBJECTIVE To establish a TLCS method for determination of camptothecin in Camptotheca acuminata fruit, and apply the method to investigate the quality of Camptotheca acuminata fruit from different producing areas. METHODS Silica gel H - CMC- Na thin layer plate was adopted in the determination with chloroform -acetone（ 7 ： 3 ） used as developer, Single -wavelength and line- ar scanning of TLC was used, and the detection wavelength was at 360 nm. RESULTS There was a good linear relationship for Camptothecin within the range of 0.0542 - 0. 3252 μg. The regression equation was Y = 97. 19X - 55.07 （ r = 0.9928 ）. The average recovery was 97.13% with RSD of 1.76% （ n = 6）. CONCLUSION The method is accurate and reliable, and the contents of camp- tothecin in Camptotheea acuminata fruit vary by different habitats.

关 键 词：喜树果 喜树碱 TLCS 含量测定 

分 类 号：R917[医药卫生—药物分析学]