L-谷氨酸与维多利亚绿S作用机理及应用研究  

Interaction between L-Glutamic Acid with Victoria Green S and Determination of L-Glutamic Acid

作　　者：陆丽园[1] 訾言勤[1] 刘本志[1] 

机构地区：[1]淮北煤炭师范学院化学系,安徽淮北235000

出　　处：《化学研究与应用》2008年第10期1268-1273,共6页Chemical Research and Application

基　　金：安徽省教育厅项目(2006kj128B)资助

摘　　要：用紫外-可见光度法、共振散射光谱法探讨了在弱酸性介质中,碱性染料维多利亚绿S与酸性氨基酸L-谷氨酸相互作用机理,认为L-谷氨酸分子通过盐键和氢键相互结合成含有亲水性基团的分子链,这条分子链可以将维多利亚绿S的疏水性基团包在里面,使其亲水性基团朝外,并通过盐键和氢键与维多利亚绿S作用形成大的聚合体,其聚合体体积远远大于染料自身聚集体的体积,使溶液颜色变深,分子光谱吸收值增加。并优化了反应体系酸度、反应物用量、反应时间、加试剂顺序等影响反应速度的因素,确定了最佳实验条件,建立了测定L-谷氨酸的方法。线性范围为10mg/L^100 mg/L,相关系数为0.9989。直接测定样品中谷氨酸获得满意结果。The interaction of L-Glutamic acid with Victoria Green S in pH 6.0 Britton-Robinson buffer solution has been investigated by UV/VIS spectrophotometry and resonance light scattering technique. It showed that L-Glutamic acids could link each other to form a long chain which contains many hydrophilic groups by salt linkages and hydrogen bonds in aqueous medium. Then the long chain surrounded the hydrophohic groups of Victoria Green S via hydrophobic interaction and reacted with Victoria Green S by salt linkages and hydrogen bonds in aqueous medium to form a macromolecular aggregation. The aggregation was much bigger than Victoria Green S itself. So the system＇ s absorption and RLS increased and its colour became deeper. This interaction has been applied to the direct determination of L-Glutamic acid by spectrophotometry. The linear range for the determination of L-Glutamic acid is from 10mg/L to 100 mg/L with correlation coefficient of 0.9989 at 620 nm. The method has been used to the determination of L-Glutamic Acid in samples with satisfactory results.

关 键 词：L-谷氨酸 维多利亚绿S 光谱法 盐键 氢键 

分 类 号：O657.3[理学—分析化学]