无机化合物CANA对大鼠血管钙化模型的改善作用  

Improvement of vascular calcification in rats by the inorganic compound CANA

作　　者：闫惠[1] 王茜倩[1] 蒲小平[1] 

机构地区：[1]北京大学药学院分子与细胞药理学系,北京100083

出　　处：《中国新药杂志》2008年第18期1584-1587,共4页Chinese Journal of New Drugs

基　　金：国家重点基础研究发展计划(973计划)资助项目(2004CB518902)

摘　　要：目的:制备大鼠血管钙化模型并观察枸橼酸盐结构类似的无机化合物CANA对血管钙化的作用。方法:用肌注维生素D3和口服尼古丁诱导大鼠血管钙化模型。4周后,进行血管组织Von Kossa染色,测定大鼠血管钙含量,碱性磷酸酶(ALP)活性、血磷、血钙含量以及心脏系数。结果:模型组大鼠血管VonKossa染色见平滑肌细胞内和细胞外基质有大量银染的深紫色颗粒聚集,主动脉血管钙含量以及ALP活性分别较对照组高66.9%(P<0.001)和462.6%(P<0.001),血磷和血钙含量及心脏系数较对照组比较无明显变化(P>0.05);无机化合物CANA740 mg.kg-1治疗组血管Von Kossa染色的深紫色颗粒有所减少,主动脉血管钙含量及ALP活性分别较模型组降低38.2%(P<0.001)和22.0%(P<0.05)。结论:用维生素D3和尼古丁制备模型4周后,可造成血管组织钙化,但血磷和血钙含量及心脏系数无明显改变。无机化合物CANA对该血管钙化模型有改善作用。Objective ： To establish the model of vascular calcification in rats, and observe the effect of the inorganic compound CANA（with similar structure to citrate） on vascular calcification. Methods： Vascular calcification in rats was induced by administration of vitamin D3 plus nicotine. Four weeks late, Von Kossa staining, cal- cium content, alkaline phosphatase aetivity, phosphorus and calcium content in blood, and heart coefficient were assayed. Results ： A strong positive staining of dark purple was found among the smooth muscle cells and the extra- cellular matrix in calcified aorta by Von Kossa staining. Calcium content and ALP activity in calcified arteries were increased by 66.9% （ P 〈 0. 001 ） and 462.6% （P 〈 0. 001 ） as compared with the controls. No significant change was tbund in phosphorus and calcium content in blood （P 〉 0.05） , and in the heart eoeffieient（P 〉 0.05）. Positive staining of Von Kossa was reduced after treatment of CANA （740 mg. kg^-1 ）. The calcium content and ALP activity in calcified arteries treated with CANA （740 mg.kg^-1 ） were dropped by 38.2% （P 〈0. 001 ） and 22.0% （P 〈 0. 05） , respectively. Conclusion： Four weeks after the treatment of vitamin D3 and nicotine, there is only significant calcification in the vascular tissue; while no significant alterations in the phosphorus and calcium contents in blood as well as the heart coefficient. The inorganic compound CANA can improve the vascular calcification in this model.

关 键 词：维生素D3 尼古丁 血管钙化 大鼠模型 无机化合物CANA 

分 类 号：R965.1[医药卫生—药理学] R972.6[医药卫生—药学]