高效液相色谱法同时测定枳壳饮片中4种黄酮类化合物的含量  

Simultaneous determination of four flavonoids in Citrus aurantium L.by HPLC

作　　者：张宏武[1] 封宇飞[2] 邹忠梅[1] 

机构地区：[1]中国医学科学院北京协和医学院药用植物研究所,北京100094 [2]卫生部北京医院药学部临床药理室,北京100730

出　　处：《中国新药杂志》2008年第18期1600-1602,1621,共4页Chinese Journal of New Drugs

基　　金：国家自然科学基金(30772711);公益性科研院所基本科研业务专项资金(YZ-1-26)

摘　　要：目的：建立高效液相色谱法测定枳壳饮片中柚皮芸香苷、柚皮苷、橙皮苷和新橙皮苷的含量。方法：采用Sunfire C18色谱柱（150 mm×4.6 mm,5μm）,以乙腈-0.1%磷酸溶液（20∶80）为流动相,流速为1 mL.min^-1,检测波长为283 nm,柱温为30℃。结果：柚皮芸香苷、柚皮苷、橙皮苷和新橙皮苷分别在0.074-123.7,0.60-999.0,0.072-120.3和0.59-982.0μg.mL^-1范围内线性良好,其平均回收率分别为96.6%,96.1%,96.0%和97.2%。结论：该方法简便、快速、准确、重复性好,可用于枳壳中柚皮芸香苷、柚皮苷、橙皮苷和新橙皮苷的同时测定。Objective：To establish a HPLC method for simultaneous determination of narirutin, naringin, hesperidin and neohesperidin in Citrus aurantium L. Methods：The HPLC analysis was performed on a Sunfire Cl8 （150 mm× 4.6 mm,5 μm）column. The column temperature was set at 30 ℃. A mixture of aeetonitrile-0. 1% phosphoric acid aqueous solution（20： 80）was used as the mobile phase with the flow rate at 1 mL-min^-1. The detection wavelength was set at 283 nm. Results：Good linearity was obtained in the ranges of 0. 074 -123.7 μg.mL^-1, 0.60 - 999.0 μg.mL^-1 , 0. 072 - 120.3 μg. mL^-1 , and 0.59 - 982.0 μg.mL^-1 with average recoveries of 96.6% ,96. 1% ,96.0% and 97.2% for narirutin, naringin, hesperidin and neohesperidin, respectively. Conclusion：The method is simple, rapid, accurate and reproducible for simultaneous determination of narirutin, naringin, hesperidin and neohesperidin in Citrus aurantium L.

关 键 词：高效液相色谱法 枳壳 柚皮芸香苷 柚皮苷 橙皮苷 新橙皮苷 

分 类 号：R927.2[医药卫生—药学]