莪术醇亚微乳剂含量及有关物质测定方法的建立  

Establishment of analytical method for the determination of curcumol and related substances in curcumol submicroemulsion

作　　者：赵晓[1] 刘玉玲[1] 

机构地区：[1]中国医学科学院药物研究所,北京100050

出　　处：《中国新药杂志》2008年第19期1684-1687,共4页Chinese Journal of New Drugs

摘　　要：目的：建立莪术醇亚微乳剂中莪术醇及有关物质的毛细管气相色谱测定方法。方法：采用气相色谱方法，HP-5柱（30．0m×0．32mm，0．25μm）；进样体积2μL，分流比10：1，进样口温度250℃；柱温（炉温）180℃，柱流量1．0mL·min^-1；检测器FID，检测器温度250℃，载气为氮气（N2）。结果：莪术醇与相共杂质及降解产物均分离良好；空白乳剂对测定无干扰。莪术醇在0．01～2．0g·L^-1范围内具有良好的线性关系（r=1．0000）；平均回收率为100．79％（RSD为1．47％）。结论：该方法用于莪术醇亚微乳剂的质量评价，操作简便，定量准确，专属性强，灵敏度高，重现性好。Objective：To establish a GC method to determine the content of eurcumol submicroemulsion and its related substances. Methods：The samples were determined by GC on a HP-5 column （30.0 m × 0.32 mm, 0.25 μm）. The injection volume was 2 μL,split ratio was 10：1 ,inlet temperature was 250 ℃. The oven temperature was 180℃. The flow rate was 1.0mL.min^-1 FID was at 250 ℃ , carrier gas was N2. Results： The good separation was obtained for curcumol and related substances. The calibration curve of curcumol was linear over the range of 0.01 -2.0g.L^-1（r= 1.0000）. The average recovery was 100.79%（RSD 1.47%）. Conclusion：The procedure of pretreatment is simple,and this method was accurate and reliable with a good reproducibility. It can be used on the quality evaluation of curcumol submicroemulsion.

关 键 词：莪术醇 亚微乳剂 含量测定 有关物质 毛细管气相色谱 

分 类 号：R286.91[医药卫生—中药学] R927.2[医药卫生—中医学]