DNA去甲基化试剂处理对青花菜春化作用的影响  

The Effect of DNA Demethylation on Vernalization in Broccoli(Brassica oleracea L. var. italica Plenck)

作　　者：于锡宏[1] 李丹[1] 蒋欣梅[1] 

机构地区：[1]东北农业大学园艺学院

出　　处：《中国蔬菜》2008年第10期16-18,共3页China Vegetables

基　　金：东北农业大学创新团队课题资助项目(CXT002-2-3);黑龙江省研究生创新科研资金项目(YJSCX2008-092HLJ);黑龙江省教育厅重点实验室(寒地蔬菜生物学实验室)--蔬菜生理与设施园艺研究室资助项目

摘　　要：以早熟品种清风103和中熟品种绿秀为试材,采用5-氮胞苷(5-azaC)不同施用方式处理,研究DNA去甲基化对青花菜春化作用和春化过程中植株体内C/N的影响。结果表明,叶面喷施5-azaC处理可明显促进青花菜春化及花芽分化,且植株体内全糖/全氮、可溶性糖/可溶性蛋白、蔗糖/可溶性蛋白的值均高于5-azaC根施处理和清水、清水+吐温对照。不同熟性品种春化启动所需的时间不同,中熟品种比早熟品种需要更长的启动时间。Taking two varieties of broccoli ‘Qingfeng 103 ＇ and ‘Luxiu＇ as materials, we adopted different 5-azaC treatments on them at young seedling stage, tested the influence of DNA demethylation on vernalization and C/N during vernalization in broccoli. The results showed that the 5-azaC spraying treatment could promote vernalization and shorten flower-bud differentiation stage, and its ratio of total sugar to total nitrogen, soluble sugar to soluble protein, sucrose to sol- uble protein were higher than the root fertilizing treatment, CK - W and CK - T.

关 键 词：DNA去甲基化 青花菜 春化作用 5-azaC 

分 类 号：S635[农业科学—蔬菜学]