苗龄与基质对黄瓜转基因组培苗驯化成活率的影响  

Impacts of Plantlet Age and Cultivation Substrates on Domestication Survival Rate of Transgenic Plantlets of Cucumis sativus L.

作　　者：缪旻珉[1] 徐冉[1] 郑丽娟[1] 周红莲[1] 

机构地区：[1]扬州大学园艺与植物保护学院

出　　处：《中国蔬菜》2008年第10期19-21,共3页China Vegetables

基　　金：江苏省高校自然科学基金重点项目(04KJA210158)

摘　　要：以黄瓜转基因组培苗为材料,研究了不同苗龄、栽培基质和移栽前培养基中的蔗糖浓度对组培苗根系发育及驯化成活率的影响。结果表明:将转基因组培苗置于蔗糖浓度为6%的1/2MS培养基中生长28d,随后移至蔗糖浓度为3%的培养基中培养7d,在组培苗六叶一心时,移栽于草炭∶珍珠岩∶河沙=1V∶1V∶1V的基质中驯化,最有利于组培苗根系的发育,驯化成活率可达98.7%,且经驯化成活的植株均能正常开花结果。Taking transgenic plantlets of Cucumis sativus L. as material, we studied the impacts of plantlet age, cultivation substrats and sucrose concentration in rooting media＇ on root development and domestication survival rate of Cucumis sativus L. transgenic in vitro culture planflets. The results showed that the best root development and the highest survival rate can be obtained when the plantlets were rooted in 1/2 MS media with 6 % sucrose for28 days and transformed to the same media with 3 % sucrose for 7 days, then transplanted in the cultivation substrates of peat ： perlite ： river sand = 1 V ： 1 V ： 1 V at 6-true leaf stage.

关 键 词：黄瓜 组培苗 驯化成活率 苗龄 基质 蔗糖浓度 

分 类 号：S642.2[农业科学—蔬菜学]