低强度HiFu辐照对体外培养神经母细胞瘤株SK-N-SH的影响及机制初步探讨  

PRIMARILY EXPLORATION OF THE EFFECT AND MECHANISM OF LOW INTENSITY HIFU ON THE SK-N-SH CELL OF NEUROBLASTOMA

作　　者：汪洋[1] 李勤[2] 

机构地区：[1]重庆医科大学公共卫生学院卫生毒理学教研室,重庆400016 [2]重庆医科大学儿童医院普外科

出　　处：《现代预防医学》2008年第20期4043-4045,共3页Modern Preventive Medicine

摘　　要：[目的]研究低强度HiFu辐照对SK-N-SH细胞的影响,并初步探讨其作用机制。[方法]MTT法检测不同的时间(10～100s)超声辐照后SK-N-SH细胞的存活率,以及超声辐照40s对SK-N-SH细胞VCR化疗敏感性的影响;扫描电镜观察超声辐照后细胞超微结构的改变。[结果]低强度HiFu对SK-N-SH细胞有细胞毒作用,辐照时间与细胞存活率之间呈现明显的剂量效应关系;超声辐照可增加细胞对长春新碱的化疗敏感性;超声辐照可导致细胞膜超微结构改变。[结论]超声辐照不仅可以杀伤SK-N-SH细胞,而且可以增强SK-N-SH细胞对长春新碱的化疗敏感性,其机制可能是通过改变细胞膜超微结构,使细胞对化疗药物通透性增加,从而发挥其杀伤效应。[Objective] To explore the tyro-toxic effect of high intensity focused ultrasound （HIFU） on SK-N-SH ceils, and to explore its possible mechanisms. [Methods] The survival rates of SK-N-SH cells were assessed by MTT assay after the cells were exposed to the HIFU irradiation of various time periods （10-100s） ; MTT assay was used to assess the effect of HIFU irradiation on the chemotherapeutic sensitivity of SK-N-SH cells; The aheration on membranes of SK-N-SH cells exposed to HIFu irradiation were analyzed by scanning electron microscope. [ Results] The low intensity HIFU irradiation had cyto-toxicity on SK-N-SH ceils, and the time of irradiation showed significant dose-response effect with the survival rate of cell; The chemotherapeutic sensitivity of SK-N-SH ceils towards VCR were increased with the HIFU irradiation; Also HIFU irradiation could induce the change of membranes of irradiated ceils. [Conclusions] SK-N-SH ceils could be killed by HIFU irradiation through cyto-toxic effect; moreover, the chemotherapeutic sensitivity of SK-N-SH cells towards VCR could be increased by HIFU irradiation. The concerned mechanism may involve in the change of uhramicrostructures on membranes of irradiated cells, which could increase the permeability of VCR into the neuroblastoma ceils to exert its anti--tumor effect.

关 键 词：HiFu(高强度聚焦超声) SK-N—SH细胞 化疗敏感性 

分 类 号：R594[医药卫生—内科学]