高效液相色谱法测定阿魏酸川芎嗪口崩片的含量  

Determination of Ligustrazine Ferulate Orally Disintegrating Tablet by HPLC

作　　者：吕洁[1] 孟祥军[2] 郭妍[1] 

机构地区：[1]辽宁中医药大学职业技术学院,辽宁沈阳110101 [2]沈阳医学院,辽宁沈阳110034

出　　处：《中国药业》2008年第20期24-25,共2页China Pharmaceuticals

摘　　要：目的建立用高效液相色谱法测定阿魏酸川芎嗪口崩片含量的方法。方法色谱柱为Diamonsil-C18柱(250mm×4.6mm,5μm),以用三乙胺调节pH=7.6的0.02mol/L磷酸二氢钾溶液-乙腈(320∶60)为流动相,检测波长320nm,流速1.0mL/min,外标法定量。结果阿魏酸川芎嗪分离完全,进样量在6.4～9.6μg范围内线性关系良好。阿魏酸线性回归方程为Y=6162969.58X-7471917.60,r=1.000;川芎嗪线性回归方程为Y=4237015.65X-3057438.64,r=1.000。阿魏酸平均回收率为99.4%,RSD=1.76%(n=9);川芎嗪平均回收率为98.9%,RSD=2.38%(n=9)。结论该方法快捷、简便、准确,可作为阿魏酸川芎嗪口崩片的含量测定方法。Objective To establish a method for determining Ligustrazine Ferulate Orally Disintegrating Tablet by HPLC. Methods Dia- monsil-C18 column（250 mm×4. 6 mm, 5 μm） was used. Acetonitrile-0.02 mol/L potassium dihydrogen phosphate（60 ： 320, with triethylamineto adjusting pH =7.6） was the mobile phase and the detection wavelength was 320 nm. Results A good linearity was obtained in the range of 6. 4 - 9. 6 μg for ferulic acid. The linear equation was Y = 6 162 969. 58X -7 471 917.60, r = 1. 000 for ligustrazine ferulate. Y = 4 237 015.65 X- 3 057 438.64, r = 1. 000 for ferulic acid. The average recovery rate of these two components： ferulaie acid 99.4%,RSD=1.76%;ligustrazine 98.9%,RSD=2.38%.Conclusion The method is simple, rapid and accurate for determining the content of Ligustrazine Ferulate Orally Disintegrating Tablet.

关 键 词：阿魏酸川芎嗪 口崩片 高效液相色谱法 

分 类 号：R927.2[医药卫生—药学] R972