小麦品种中梁88375抗条锈病基因的分子作图  

Molecular Mapping of Stripe Rust Resistance Gene in Wheat Line Zhongliang88375

作　　者：杨敏娜[1,2] 徐智斌[3] 王美南[1] 宋建荣[4] 井金学[1] 李振岐[1] 

机构地区：[1]西北农林科技大学植物保护学院/陕西省农业分子生物学重点实验室,陕西杨凌712100 [2]西藏农牧学院,西藏林芝860000 [3]中国科学院成都生物研究所,成都610041 [4]甘肃省天水市农业科学研究所,甘肃天水741001

出　　处：《中国农业科学》2008年第10期2931-2936,共6页Scientia Agricultura Sinica

基　　金：国家“973”项目(G2000016200);教育部长江学者和创新团队发展计划(200558);陕西省科技攻关项目(2005k02-G1-1)

摘　　要：【目的】中梁88375是甘肃省天水市农业科学研究所以中4/S394//咸农4号复合杂交选育而成的冬小麦品系,对小麦三锈免疫。明确其抗条锈病基因及遗传特点,建立与其连锁的微卫星标记,以利于抗源筛选和培育持久抗病新品种。【方法】将中梁88375与感病品种铭贤169杂交、自交和测交并对双亲及其杂交后代进行苗期抗性鉴定。用小麦条锈菌条中31号对其进行遗传分析;采用SSR技术,选用普通小麦的320对微卫星引物对中梁88375及铭贤169的基因组DNA进行PCR扩增和电泳分析。【结果】中梁88375对多个条锈菌小种具有良好的抗病性,对CY31的抗病性由1对显性核基因控制,把该基因暂命名为Yr88375。建立了与Yr88375连锁的6个微卫星标记Xgwm335、Xwmc289、Xwmc810、Xgdm116、Xbarc59与Xwmc783,并将Yr88375定位于小麦5BL。距离Yr88375最近的两个微卫星位点是Xgdm116、Xwmc810,遗传距离分别是3.1cM和3.9cM,最远的标记Xwmc783与Yr88375之间的遗传距离为13.5cM。【结论】系谱分析结合分子标记结果表明,Yr88375很有可能是一个来自中间偃麦草(E.intermedium)并与已知抗条锈病基因不同的新基因。[ Objective ] Stripe rust is one of the most important diseases of wheat worldwide. Zhongliang88375, a winter wheat line highly resistant to three rusts of wheat, was developed from a cross of Zhong4/S394//Xiannong4 made by the Institute of Agricultural Science of Tianshui. Inheritance of stripe rust resistance gene and mapping of that in Zhongliang88375 with simple sequence repeat （SSR） markers were studied to formulate efficient strategies for breeding cultivars resistant to stripe rust. [Method] F1, F2 and BC1 populations were constructed on the basis of the hybridization between Zhongliang 88375 and a susceptible wheat line Mingxian 169; 320 SSR primer pairs were used for analyzing the genetic linkage relation. [Result] Genetic analysis of Zhongliang 88375 indicated that the resistance to PST race CY31 was controlled by a single dominant gene, temporarily designated as Yr88375. Six SSR markers, Xgwm335, Xwmc289, Xwmc810, Xgdm116, Xbarc59, and Xwmc783, are linked to Yr88375 as they were all located on chromosome 5BL. Yr88375 was also located on that chromosome arm, closely linked to Xgdm116 and Xwmc810 with genetic distances of 3.1 and 3.9 cM, respectively. The farthest marker Xwmc783 was 13.5 cM to Yr88375. [ Conclusion ] Hence, pedigree analysis of Zhongliang 88375 combined with SSR markers supports the conclusion that the highly resistance gene Yr88375 derived from Elytrigia intermedium is a novel gene for resistance to stripe rust in wheat. It could play an important role in wheat breeding programs for stripe rust resistance.

关 键 词：小麦条锈病 中梁88375 抗病基因 分子作图 

分 类 号：S512.1[农业科学—作物学] U272.306[机械工程—车辆工程]