转PEPC＋PPDK双基因水稻的光合特性  

Photosynthetic Characteristics of Transgenic Rice with PEPC+PPDK Gene

作　　者：张边江[1,2] 华春[2] 周峰[2] 周泉澄[2] 陈全战[2] 王荣富[1] 焦德茂[3] 

机构地区：[1]安徽农业大学生命科学学院,合肥230036 [2]南京晓庄学院生命科学系,南京211171 [3]江苏农业科学院生物技术所,南京210014

出　　处：《中国农业科学》2008年第10期3008-3014,共7页Scientia Agricultura Sinica

基　　金：国家转基因专项(16100004);安徽省自然科学基金项目(01041106)

摘　　要：【目的】系统研究ATP处理后转PEPC+PPDK双基因水稻的光合特性,证明ATP是增强转C4基因水稻光合能力的关键因子。【方法】以原种和转PEPC+PPDK双基因水稻为材料,进行了PCR检测,C4光合酶活性的测定。通过ATP处理后,分析了光、温—光合曲线和活性氧代谢有关指标,统计分析了相关的产量构成因素。【结果】原种中虽有全套的C4光合酶,但活性很低。PCR检测出玉米的PEPC和PPDK基因转入普通水稻后,转PEPC+PPDK双基因水稻高表达了C4光合酶活性。在高光和高温条件下,同未施ATP的相比,ATP处理后转PEPC+PPDK双基因水稻光合速率分别提高17%和12%。在光氧化条件下,耐光氧化能力进一步增强,产量提高15%。【结论】ATP处理后,转PEPC+PPDK双基因水稻增强了光合生产力,表明ATP是设计类似C4水稻的关键因子。[Objective] The aim of this paper is to study photosynthetic characteristics of PEPC＋PPDK transgenic rice for demonstrating that ATP is the key factor enhancing the photosynthetic capacity in transgenic rice with C4 genes. [ Method ] With the untransformed rice and PEPC＋PPDK transgenic rice as the materials, PCR test and the activity of C4 photosynthetic enzymes were conducted. After the ATP treatment, the light, temperature-photosynthetic curves, the parameters related to the metabolism of active oxygen and the component factors of yield were analyzed. [ Result ] The untransformed rices posses all the C4 photosynthetic enzymes but their activities are very low. The PCR test indicated that PEPC and PPDK gene from maize were transformed into common rice. The activities of the C4 enzymes in PEPC＋PPDK transgenic rice were highly expressed. Under the conditions of high photon flux density and high temperature, the photosynthetic rate of PEPC＋PPDK transgenic rice after the ATP treatment increased by 17% and 12%, respectively, compared with the controle without ATP treatment. The resistance of PEPC＋PPDK transgenic rice against photooxidation was further enhanced under the photooxidative conditions. The yield of PEPC＋PPDK transgenic rice were also increased by 15%. [Conclusion] The photosynthetic productivity of PEPC＋PPDK transgenic rice after the ATP treatment was enhanced, demonstrating that ATP is the key factor for designing the C4-like rice.

关 键 词：转基因水稻 光合特性 C4光合途径 ATP 

分 类 号：S511[农业科学—作物学]