钙黄绿素-钯(Ⅱ)荧光光度法测定土壤中甲拌磷残留  

Fluorometric Determination of Phorate with Calcein-Pd(Ⅱ) in Soil

作　　者：张明丽[1] 董学芝[1] 张国胜[1] 王明瑞[1] 

机构地区：[1]河南大学化学化工学院污控所,河南开封475001

出　　处：《分析科学学报》2008年第5期583-585,共3页Journal of Analytical Science

摘　　要：基于钙黄绿素在pH6．4的NaOH-KH2PO4缓冲溶液中有荧光，钯（Ⅱ）与钙黄绿素反应使其荧光猝灭，甲拌磷与钙黄绿素-钯（Ⅱ）反应置换出钙黄绿素，重显荧光，分别以493nm和514nm为激发波长和发射波长，建立了一种测定甲拌磷的新方法。方法的线性范围为2．0×10^-8～2．0×10^-7mol·L^-1，相关系数为0．9988，检出限为5．3×10^-9mol·L^-1用该法对土壤样品进行分析，并与色谱法对照，结果比较满意。In this study, we proposed a novel method to determine the trace phorate in soil. It is based on the fluorescence of the calcein in the pH 6. 4 NaOH-KH2PO4 buffer solution and the fluorescence quenching by Pd（ Ⅱ ）. Since Pd（ Ⅱ） complexes much stronger to the phorate than calcein does, the reaction between phorate and calcein-Pd （Ⅱ） can replace calcein, accordingly, the fluorescence is observed. Taking 493 nm and 514 nm as the excitation and emission wavelengths, respectively, the linear range is 2.0 × 10^-8 -2.0 × 10^-7 mol· L^-1 and the detection limit is 5.3 × 10^-9 mol·L^-1. The soil sample is analysed with this method. Compared to the chromatographic measurement, the result is satisfied.

关 键 词：荧光光度法 甲拌磷 钙黄绿素 

分 类 号：O657.39[理学—分析化学]