化学衍生与萃取联用技术在生物样品预处理中的应用  

Applications of Extraction Technique Combined with Chemical Derivatization in Biological Sample Preparation

作　　者：张慧娟[1] 吴剑虹[1] 冯钰锜[1] 

机构地区：[1]武汉大学化学与分子科学学院,武汉430072

出　　处：《分析科学学报》2008年第5期589-594,共6页Journal of Analytical Science

基　　金：国家杰出青年科学基金(No.20625516)

摘　　要：近年来各种萃取技术与化学衍生联用的模式作为一种新型样品处理技术广泛应用于生物样品预处理。这种联用技术不仅能有效去除样品基质,还能有效改善极性大、挥发性强、热稳定性差的目标分析物的回收率,提高方法的选择性和灵敏度。研究者根据待分析物及衍生试剂性质选取不同的联用模式。本文评述了萃取技术与化学衍生联用模式以及其在生物样品预处理中的应用。Various extraction techniques combined with chemical derivatization emerges as a novel sample preparation technique in recent years. This novel technique not only removes the sample matrix from samples, but also increases the affinity of volatile, polar or thermally unstable analytes with extraction media to achieve better detectability and recoveries at trace levels. Researchers adopt different combination modes according to the characters of analytes and derivatization reagents. This review focuses on the operation modes and applications of extraction technique combined with chemical derivatization in biological sample preparation.

关 键 词：衍生 萃取技术 样品预处理 

分 类 号：O652.62[理学—分析化学]