根癌农杆菌介导的水稻遗传转化体系探索  

Study on the Genetic Transformation System Mediated by Agrobacterium Tumefaciens in Rice

作　　者：尹梅[1] 张伟媚[1] 程在全[2] 陈善娜[1] 

机构地区：[1]云南大学生命科学学院生物技术系,云南昆明650091 [2]云南省农业科学院,云南昆明650205

出　　处：《贵州农业科学》2008年第5期7-9,共3页Guizhou Agricultural Sciences

基　　金：云南省农业生物技术重点实验室开放基金项目"甘油-3-磷酸转酰酶全基因克隆;转化和分析"(2001A01)

摘　　要：对根癌农杆菌介导的水稻遗传转化受体系统的几个重要因素进行试验。得到157个抗性愈伤组织。对于滇优粳3号和滇优粳5号，4mg／L的2，4-D和0．2mg／L的6-BA搭配，蔗糖浓度为20～30g／L时比较有利于愈伤组织形成。滇优粳3号和滇优粳5号的愈伤组织对潮霉素较敏感，30～50mg／L的靓霉素浓度为有效的筛选浓度。Two rice varieties, Dianyoujing 3 and Dianyoujing 5, were tested to study some important factors affecting rice genetic transformation system mediated by agobacterium tumefaciens and 157 callus with Hyg resistance were obtained. The results showed that the callus induction rate of Dianyoujing 3 and Dianyoujing 5 was 72.50% and 71.21% under the culture conditions of MS＋ 4 mg/L 2, 4-D, 0.2 mg/L 6-BA and 20～30 g/L cane sugar respectively. The optimum hygromyein concentration for selecting callus with Hyg resistance was to 30～50 mg/L in Dianyoujing 3 and Dianyoujing 5.

关 键 词：水稻 转化 因素 根癌农杆菌 

分 类 号：S511.032[农业科学—作物学] Q943.2[生物学—植物学]