RP-HPLC法测定半乳糖化肝靶向葫芦素B脂质体中药物含量  

Content determination of cucurbitacin B in galactosylated hepatocyte-targeting liposomes by RP-HPLC

作　　者：董晓辉[1] 刘欣荣[2] 石莉[1] 卢懿[1] 徐缓[1] 邓意辉[1] 

机构地区：[1]沈阳药科大学药学院,辽宁沈阳110016 [2]沈阳药科大学高等职业技术学院,辽宁沈阳110026

出　　处：《沈阳药科大学学报》2008年第10期816-819,共4页Journal of Shenyang Pharmaceutical University

摘　　要：目的建立半乳糖化肝靶向葫芦素B脂质体中药物含量测定的方法，对制剂进行有效的质量控制。方法采用RP—HPLC法。色谱柱为Diamonsil C18柱（200mm×4．6mm，5μm），流动相为乙腈-水（体积比50：50），流速1mL·min^-1，检测波长233nm，柱温35℃。结果在本色谱条件下，半乳糖化肝靶向脂质体辅料对葫芦素B的测定无干扰，葫芦素B质量浓度在1．0～20mg·L^-1内与峰面积呈良好的线性关系（r=0．9999，n=5），葫芦素B平均回收率为100．6％，RSD为1．0％（n=9）。结论本法准确、可靠、简便、快速，可用于半乳糖化肝靶向葫芦素B脂质体中药物含量的质量控制。Objective To establish an RP-HPLC method for the determination of eueurbitaein B in galactosylated hepatocyte-targeting liposomes. Methods The separation was performed by HPLC with a Diamonsil C18 reversed-phase column （200 mm×4.6 mm, 5 μm） at 35 ℃ with a mobile phase consisting of acetonitrile-water （ V ： V = 50 ： 50）. The flow rate was 1.0 mL·min^-1 and the effluent was monitored photometrically at 233 nm for the determination of cucurbitacin B. Results Cucurbitacin B had a good linear relation over the range of 1.0- 20 mg·L^-1（ r = 0. 999 9, n = 5）. The average recovery of cucurbitacin B was 100.6 % and RSD was 1.0 % （ n = 9）. Conclusions An accurate and simple method is established for the determination of cucurbitacin B in galactosylated hepatocyte-targeting liposomes.

关 键 词：葫芦素B 半乳糖化 肝靶向 脂质体 反相高效液相色谱法 含量测定 

分 类 号：R917[医药卫生—药物分析学]