正交设计法优选坎离砂的提取工艺  

Selection of optimum extraction process for Kanlisha by orthogonal design

作　　者：郭允[1] 赵春杰[2] 万绍晖[1] 洪博[2] 李丹[2] 曲龙妹[2] 

机构地区：[1]河南大学药学院,河南开封475001 [2]沈阳药科大学药学院,辽宁沈阳110016

出　　处：《沈阳药科大学学报》2008年第10期820-824,共5页Journal of Shenyang Pharmaceutical University

摘　　要：目的优选坎离砂的提取工艺，以提高坎离砂的疗效，降低用药剂量，保证临床疗效的稳定性。方法以坎离砂的浸膏得率，升麻苷、川芎嗪、阿魏酸的含量为指标，比较坎离砂在水、醋和乙醇溶液中的提取工艺，并用L9（3）^4正交实验优化醇提工艺；用RP—HPLC法同时测定升麻苷、川芎嗪、阿魏酸含量。结果最佳提取工艺为：坎离砂用8倍量的质量分数为80％的乙醇提取2次，每次1h；升麻苷质量浓度在8．440-135．1mg·L^-1内、川芎嗪质量浓度在6．910～158．0mg·L^-1内、阿魏酸质量浓度在14．35～328．0mg·L^-1内与峰面积呈良好的线性关系，升麻苷、川芎嗪、阿魏酸平均回收率分别为99．86％、99．21％、99．24％，RSD分别为1．2％、1．2％、1．2％。结论优选后的提取工艺适用于大生产；RP—HPLC法可用于坎离砂及其制剂的质量控制。Objective To find out the optimum extraction process for Kanlisha （traditional Chinese medicines）. Methods The yield of extracts and the contents of cimicifugoside, chuanxiongzine and ferulic acid were taken as the assessment index, and HPLC was used for simultaneous determination of three effective ingredients. Different extraction processes with different solvents （water, vinegar, ethanol） were compared, and the third process was optimized with orthogonal design. Results The optimum extraction process is：Adding 8 times amount of 80% （V： V） ethanol, and extracting for two times, 1 h each time. Conclusions The optimum extraction process is reasonable, stable and fit for a large production.

关 键 词：坎离砂 正交设计 高效液相色谱法 含量测定 

分 类 号：R917[医药卫生—药物分析学] R966[医药卫生—药学]