Effects of starvation on the vitellogenesis, ovarian development and fecundity in the ectoparasitoid, Nasonia vitripennis （Hymenoptera： Pteromalidae）  

作　　者：Sheng-Zhang Dong Gong-Yin Ye Peng-Cheng Yao Yao-Liang Huang Xue-Xin Chen Zhi-Cheng Shen Cui Hu 

机构地区：[1]State Key Laboratory of Rice Biology, Institute of Insect Sciences, Zhejiang University, Hangzhou [2]College of Life Sciences, China Jiliang University, Hangzhou [3]Jiangxi University of Traditional Chinese Medicine, Nanchang, Jiangxi Province, China

出　　处：《Insect Science》2008年第5期429-440,共12页昆虫科学（英文版）

基　　金：Acknowledgments This work was supported by the National Basic Research and Development Program of China （2006CB 102005）, the National Natural Science Foundation of China （Grant No. 30270899）, the program for New Century Excellent Talents in the University of the Ministry of Education of China （NCET-05-0513）, and the Innovation Research Team Program of the Ministry of Education of China （Grant No. IRT0535）.

摘　　要：A female-specific protein, vitellogenin （Vg）, and its corresponding egg vitellin （Vt） are identified in the ectoparasitic wasp Nasonia vitripennis. Both native Vt and Vg have a molecular mass of about 350 kDa, which is composed of two subunits of approximately 190 kDa and 165 kDa under reducing and denaturing conditions （sodium dodecyl sulfate-polyacrylamide gel electrophoresis）. An indirect sandwich enzyme-linked immunosorbent assay developed with both monoclonal and polyclonal antibodies against N. vitripennis Vt. Vg was first detected in the hemolymph on the 10th day after parasitism, and was first observed in oocytes on the 12th day. In adults deprived of food, the highest hemolymph Vg level occurred at the time of adult eclosion and the highest level of Vt in ovaries was found at 30 h after eclosion. In contrast, feeding adults with 20% sucrose resulted in the reduction of Vt uptake by ovaries and the extension of life span, but had little effect on Vg production. Deprived of hosts, starvation of female wasps had no significant effects on ovariole growth and oocyte maturation before the wasps died. However, starvation of female wasps supplied with hosts accelerated the wasps laying progeny into hosts, but resulted in a decrease of total progeny production by comparison with wasps fed with 20% sucrose.A female-specific protein, vitellogenin （Vg）, and its corresponding egg vitellin （Vt） are identified in the ectoparasitic wasp Nasonia vitripennis. Both native Vt and Vg have a molecular mass of about 350 kDa, which is composed of two subunits of approximately 190 kDa and 165 kDa under reducing and denaturing conditions （sodium dodecyl sulfate-polyacrylamide gel electrophoresis）. An indirect sandwich enzyme-linked immunosorbent assay developed with both monoclonal and polyclonal antibodies against N. vitripennis Vt. Vg was first detected in the hemolymph on the 10th day after parasitism, and was first observed in oocytes on the 12th day. In adults deprived of food, the highest hemolymph Vg level occurred at the time of adult eclosion and the highest level of Vt in ovaries was found at 30 h after eclosion. In contrast, feeding adults with 20% sucrose resulted in the reduction of Vt uptake by ovaries and the extension of life span, but had little effect on Vg production. Deprived of hosts, starvation of female wasps had no significant effects on ovariole growth and oocyte maturation before the wasps died. However, starvation of female wasps supplied with hosts accelerated the wasps laying progeny into hosts, but resulted in a decrease of total progeny production by comparison with wasps fed with 20% sucrose.

关 键 词：Nasonia vitripennis oocyte starvation VITELLIN VITELLOGENIN 

分 类 号：Q969.2[生物学—昆虫学]