口腔白斑癌变过程中整合素β1的表达及其与细胞增殖的关系  

Expression of integrin-β1 in oral leukoplakia and early invasive carcinoma and its relationship with cell proliferation

作　　者：尚建伟[1] 高岩[1] 

机构地区：[1]北京大学口腔医学院·口腔医院口腔病理研究室,100081

出　　处：《中华口腔医学杂志》2008年第10期608-611,共4页Chinese Journal of Stomatology

摘　　要：目的探讨整合素β1在口腔白斑癌变发生、发展中的可能作用及其与细胞增殖的关系。方法免疫组化SP法研究整合素B1及Ki-67在12例正常口腔黏膜、10例单纯增生白斑、24例异常增生白斑、14例早期浸润癌组织中的表达情况及关系。结果在正常黏膜及单纯增生白斑中整合素β1表达于基底及副基底层细胞的胞膜；Ki-67在基底及副基底层的胞核中表达。在异常增生白斑及早期浸润癌中可见整合素β1表达于基底层、增生的棘层细胞及癌细胞胞膜；Ki-67表达于基底层、增生的棘层细胞及癌细胞的胞核。整合素β1在异常增生及早期浸润癌中的强阳性表达率分别为29％（7／24）、57％（8／14）；Ki-67在异常增生及早期浸润癌中的强阳性表达率分别为42％（10／24）、57％（8／14）。整合素β1、Ki-67在4组病变之间的阳性分级差异有统计学意义（x^2=10．651，P=0．014；x^2=14．831，P=0．002）；整合素β1在正常黏膜、单纯增生组与早期浸润癌组之间的差异有统计学意义（P=0．008，P=0．013）。Ki-67在正常黏膜组与异常增生组、早期浸润癌组之间差异有统计学意义（P=0．026，P=0．001），单纯增生组与早期浸润癌组之间差异有统计学意义（P=0．005）。整合素β1与Ki-67的表达显著相关（R=0．442，P〈0．01）。结论整合素β1在口腔白斑异常增生及早期浸润癌中表达增强，可能与促进细胞增殖有关。Objective To investigate the expression and significance of integrin-β1 in oral leukoplakia and early invasive carcinoma. Methods The expression of integrin-β1 and Ki-67 was examined in 12 normal oral mucosa, 10 simple hyperplasia ,24 epithelial dysplasia and 14 early invasive carcinoma by immunohistochemistry. Results In normal and simple hyperplasia epithelium, integrin-β1 was mostly expressed in the basal cell membrane, and Ki-67 in the nuclei of basal and the parabasal layers. In dysplasia epithelium and early invasive carcinoma, integrin-β1 showed membrane staining and Ki-67 showed nuclear staining in dysplastic basal cells, sprickle cells and SCC cells. Integrin-β1 and Ki-67 were overexpressed in 7 and 10 of 24 dysplasia cases and in 8 of 14 early invasive carcinoma cases respectively. There was a significant difference in integrin-131 and Ki-67 expression among the four groups（ X^2 = 10. 651 ,P = 0. 014; X^2 = 14. 831 ,P=0. 002） ,in integrin-β1 expression among normal oral mucosa,simple hyperplasia and early invasive carcinoma （ P = 0. 008, P = 0. 013 ） and in Ki-67 expression among normal oral mucosa and dysplasia, early invasive carcinoma （ P = 0. 026, P = 0. 001 ）, and among simple hyperplasia and early invasive carcinoma （ P = 0. 005 ）. A significant correlation between integrin-β1 and Ki-67 （ R = 0. 442, P 〈 0. 01 ）was found. Conclusions Integrin-β1 showed increased staining in dysplasia epithelium cells and SCC cells, and may correlate to the proliferation of the dysplasia cells.

关 键 词：白斑 口腔 抗原 CD29 细胞增殖 早期浸润癌 

分 类 号：R686[医药卫生—骨科学]