成纤维生长因子(FGF2)和成纤维生长因子受体(FGFR1)在新生死亡体细胞克隆牛组织中的表达  

Expression of Fibroblast Growth Factors(FGF2) and Fibroblast Growth Factor Receptors(FGFR1) in Organs of Cloned Cattle that Die within Hours after Birth

作　　者：李世杰[1,2] 李冬杰[3] 杜卫华[4] 樊宝良[1,2] 戴蕴平[2] 李宁[2] 

机构地区：[1]河北农业大学生命科学学院,保定071001 [2]中国农业大学农业生物技术国家重点实验室,北京100193 [3]河北科技大学生物科学与工程学院,石家庄050018 [4]中国农业科学院北京畜牧兽医研究所,北京100193

出　　处：《畜牧兽医学报》2008年第10期1289-1294,共6页ACTA VETERINARIA ET ZOOTECHNICA SINICA

基　　金：国家“863”计划生物工程主题项目(2001AA213091);河北省自然科学基金项目(C2006001032)资助

摘　　要：为了探讨成纤维生长因子及其受体在克隆动物出生死亡以及器官发育异常中的可能作用,本研究用荧光定量RT-PCR技术分析了成纤维生长因子基因(FGF2)及成纤维生长因子受体基因(FGFR1)在来自2种供体细胞(成年成纤维细胞和胎儿成纤维细胞)的出生死亡克隆牛的6个组织(心脏、肝脏、脾脏、肺脏、肾脏和大脑)中的表达。结果表明:FGFR1的表达在来自两种供体细胞的体细胞克隆牛的心脏(P<0.05)和肝脏(P<0.05)显著升高,FGF2基因在体细胞克隆牛组织中的表达未发现异常。由于FGFR1在胚胎发育和器官形成中起重要作用,所以FGFR1的异常表达可能是造成克隆动物出生死亡以及器官发育异常的原因之一。Cloning by somatic nuclear transfer is an inefficient process in which many of the cloned animals die shortly after birth and display organ abnormalities. In an effort to determine the possible roles of FGFs played in neonatal death and organ abnormalities, we have examined expression patterns of fihroblast growth factor （FGF2） and their receptor （FGFR1） in six organs （heart, liver, spleen, lung, kidney and brain） of both neonatal death cloned bovines （n=9） and normal control calves （n=3） produced by artificial insemination （AI） using real time quantitative RT PCR. The effect of the age of the fibroblast donor cell on the gene expression profiles was also investigated. Aberrant expressions of FGFR1 were found in some studied tissues, but the expression of FGF2 had similar levels with the normal controls. The expression of FGFR1 showed a higher level in heart and liver of both cloned bovines. Because FGF systems play an important role in embryo development and organogenesis, the aberrant transcription patterns detected in these clones may contribute to the defects of organs reported in neonatal death of clones.

关 键 词：体细胞克隆 器官异常 新生死亡 成纤维生长因子 基因表达 

分 类 号：S823.2[农业科学—畜牧学]