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作 者:杨竹青[1] 陈从英[1] 任军[1] 郭源梅[1] 杨广成[1] 麻骏武[1] 高军[1]
机构地区:[1]江西农业大学动物生物技术国家重点实验室培育基地,南昌330045
出 处:《畜牧兽医学报》2008年第10期1306-1311,共6页ACTA VETERINARIA ET ZOOTECHNICA SINICA
基 金:973项目(2006CB102103)
摘 要:本研究根据FBJ骨肉瘤原癌基因同源物B(FosB)在动物母性行为发生过程中的重要作用,选择其作为影响母猪母性行为的候选基因,以白色杜洛克×二花脸F2资源群体为材料,分析了FosB基因在此群体中的遗传变异及其与分娩母猪母性行为的相关性。采用PCR产物直接测序法搜寻了FosB基因4888、06 bp 2个片段的单核苷酸多态位点(SNPs),鉴别到FosB-EU163403-g.187A>T,FosB-EU163403-g.188A>C和FosB-EU163403-g.297C>G 3个SNPs。采用PCR-MbiI-RFLP法分析了G297C位点在资源群体中的多态及与母猪母性行为的相关性。传递不平衡检测(TDT)表明该多态位点与母猪杀婴行为、衔草做窝、哺乳、踩仔猪、压仔猪等母性行为均无显著相关性(P>0.364)。FBAT分析结果也证实了这一点(P>0.236)。考虑到母性行为属于复杂性状,可能由微效多基因控制,因此有必要在更大规模群体进行分析或搜寻其他候选基因的突变位点。In this study, FosB was chosen as a candidate gene affecting sow maternal behaviours for its important role on process of animal maternal behaviours. Genetic variations of FosB and their association with sow maternal behaviours were analyzed in a White Duroc X Erhualian resource population. Single nucleotide polymorphisms (SNPs) were screened in two FosB ampli-ons of 488 and 806 bp by directly sequencing the PCR products. Three SNPs including FosB- EU163403-g. 187A〉T, FosB-EU163403-g. 188A〉C and FosB-EU163403-g. 297C〉G were identified. The association of the g. 297C〉G polymorphism with sow maternal behaviours was evaluated in the White Duroc× Erhualian intercross using PCR-Mbi I-RFLP. Transmittion disequilibrium test (TDT) results showed that there was no significant association between this polymorphism and sow maternal infanticide, nest building, nurturing, piglets squashing and trampling (P〉0. 364). The result was confirmed by Family-Based Association Tests (FBAT, P〉0. 236). Considering that maternal behaviours are complex traits and controlled by polygene, further analysis in a larger population and (or) in other candidate genes are worthwhile.
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