PLGA微球包裹生长抑素融合基因表达质粒对家兔生长的影响  

Effects of Somatostatin Fusion Gene Carried with PLGA Microspheres on Rabbits Growth

作　　者：戴建威[1,2] 章倩倩[3] 王妍[4] 刘松财[2] 张永亮[5] 

机构地区：[1]广州医学院生化教研室,广州510182 [2]吉林大学畜牧兽医学院,长春130062 [3]中山大学生物工程中心真核分子生物学实验室,广州510275 [4]延边大学农学院动物医学系,龙井133400 [5]华南农业大学动物科学学院,广州510642

出　　处：《畜牧兽医学报》2008年第10期1415-1420,共6页ACTA VETERINARIA ET ZOOTECHNICA SINICA

基　　金：国家自然科学基金(3027097330571335)

摘　　要：将HBsAg与Somatostatin（SS）融合基因克隆到真核表达载体pIRESlneo—CMV，构建了pIRESlneo—HBsAg／SSM。应用脂质体介导转染CHO细胞，96h提取细胞总RNA，RT-PCR鉴定融合基因表达；并收集细胞上清，ELISA法测定上清中HBsAg表达情况，结果为阳性。大量提取pIRESlneo—HBsAg／SSM质粒，用Poly D，L—lactide co—glycolicacid（PLGA）微球包裹，注射Vc獭兔后肢胫前肌，以注射生理盐水獭兔为对照。动物常规饲养45d，观察日增重并测定血清中IGF-Ⅰ、SS含量以及抗HBsAg抗体水平。结果显示注射后15～30d，质粒注射组日增重较对照组高106．8％（P〈0．01）；30d时，血清中IGF-Ⅰ浓度质粒组比对照组高29．2％（P〈0．05）；检测整个过程中血清中SS含量，发现注射后30d时明显低于对照组，且出现整个试验过程的最低值；质粒注射组血清中抗HBsAg抗体水平在注射后30d时出现峰值，这与SS含量出现最低值相吻合。本试验结果表明HBsAg与SS融合基因免疫獭兔，可以促进动物生长。In this paper, we described the regulation of animal growth using plasmid DNA encoding the SS gene that fused with the hepatitis B surface antigen （HBsAg） gene. The fusion genes were successfully expressed in CHO cells, as proven by RT-PCR and ELISA assayed. Poly D, L-lactide-co-glycolic acid （PLGA） plasmid encapsulating microspheres were prepared and injected directly into the anterior tibialis of rabbits. Daily weight gain and the levels of insulinlike growth factor-Ⅰ （IGF-Ⅰ）, SS, and hepatitis B surface antibody （HBsAb） were monitored. From days 15 to 30 postinjection, the daily weight gain of the treated group was significantly increased （P〈0.01） by 106.8%. A significant increase in the IGF-Ⅰ concentration and decrease in the SS level of the treated group were also observed. The results indicated that the combination of plasmidmediated positive immunization against SS led to more robust weight gain in rabbits.

关 键 词：生长抑素 核酸免疫 乙肝表面抗原 PLGA 免疫中和 

分 类 号：S852.2[农业科学—基础兽医学]